Clinical Significance Of Expression Of PDGFR And NF-κB In Gastric Carcinoma Patients

Objective: To assess the prognostic significance of PDGFR and NF-κB in human gastric carcinoma tissue.Methods: A total of 183 human gastric carcinoma tissues and their adjacent tissues from surgical specimens and 30 normal gastric mucosal tissues from endoscopic biopsies were collected, and the protein levels of PDGFR and NF-κB were determined by immunohistochemistry(IHC). The correlation between the expression levels and patient clinicopathological characteristics and prognosis was also analyzed.Results: Among 183 patients who underwent radical resection of gastric carcinoma, 114 survived, 69 died, the median survival time was 35.6 months(3.1-68.7 months), the survival rates at 1, 3, 5 year after surgery were 79.2%, 50.8%, 40.3% respectively. IHC results revealed that PDGFR mainly located in cytoplasm and little on the membrane. The positive expression rate of PDGFR was about 52.5%(96/183), and high expression rate was about 33.9%(62/183) in the carcinoma tissues; where in the tumor adjacent tissues, the positive expression rate of PDGFR was only 8.7%(16/183)(P < 0.05). However, in the normal gastric mucosa, the expression of PDGFR was negative. Statistic analysis showed that the up-regulated expression of PDGFR was associated with tumor size, tumor differentiation, invasive depth, lymph node metastasis and the TNM stageing. Similarly, NF-κB mainly located in cytoplasm, little in the nuclei. The positive expression rate of NF-κB was about 49.7%(91/183), high expression rate of NF-κB was about 30.6%(56/183) in the carcinoma tissues. Compared with gastric cancer tissue, NF-κB positive expression rate in tumor adjacent tissues was only 6%(11/183)(P < 0.05). In the normal gastric mucosa, the expression of NF-κB was also negative. The up-regulated expression of NF-κB was associated with tumor size, tumor differentiation, invasive depth, lymph node metastasis and the TNM staging. Spearman correlation analysis indicated that in the gastric carcinoma, the expression of PDGFR was a positive correlation with the expression of NF- kappa B(P < 0.001). A multivariate analysis indicated that tumor size, invasive depth, lymph node metastasis, postoperative adjuvant chemotherapy, and the up-regulated expression of PDGFR and NF-κB were the independent risk factors for the postoperative prognosis of gastric carcinoma.Conclusion: The expression of PDGFR and NF-κB in gastric carcinoma tissue was up-regulated and associated with tumor size, tumor differentiation, invasive depth, lymph node metastasis and the TNM staging. There is a synergistic effect of PDGFR and NF-κB during the development and progression of gastric cancer. The up-regulated expression of PDGFR and NF- kappa B in gastric carcinoma tissue is one of the independent risk factors for the prognosis of the patients with gastric carcinoma.