| Objective:Observe the effect on the serum IGF-1, VEGF and clinical efficacy of DanShen after rhG-CSF mobilization of ischemic stroke patients at recovery stage. Exploring the probably approach of DanShen inducing stem cell differentiation.Methods:Using a randomized, single-blind, parallel-group clinical trial,60inpatients allowed were randomly divided into four groups:the conventional treatment group, the Danshen group, the rhG-CSF group, the Danshen+rhG-CSF group,15inpatients in each group. The Danshen+rhG-CSF group, the G-CSF group, the Danshen group,the blank control group,15inpatients in each group. the Danshen group was treated with Salvia miltiorrhiza injection,the rhG-CSF group Subcutaneous injection of rhG-CSF, the Danshen+rhG-CSF group was combined with intravenous infusion of Salvia miltiorrhiza injection with Subcutaneous injection of rhG-CSF, more than three above groups deal with the conventional treatment group given based therapy+rehabilitation training. Clinical observation of3weeks. Detection and comparison of serum IGF-1, VEGF changes, clinical efficacy was evaluated based on the NIHSS score, modified Barthle index, the effect of TCM, compared before and after the treatment among groups.Results:1) Itself before and after treatment:Danshen+rhG-CSF group and rhG-CSF group serum IGF-1, VEGF levels rise, the difference was statistically significant (P<0.05); the serum IGF-1content of Danshen group and conventional treatment group, had no significant difference; Danshen group serum VEGF had a significant difference (P<0.05).2) Between the two groups after treatment:the serum IGF-1content of Danshen+rhG-CSF group more than the other three groups, the difference was statistically significant (P<0.05). Comparing the serum VEGF of Danshen+rhG-CSF group and Danshen group, the difference was not statistically significant, but with the rhG-CSF group or the routine treatment group, the difference was statistically significant (P<0.05).3) Comparison of clinical efficacy:Danshen+rhG-CSF group, NIHSS score, symptom score lower than the other three groups, the modified Barthle index higher than the other three groups, the difference was statistically significant (P<0.01). Conclusions:1. RhG-CSF applicated separately to mobilize stem cells can promote the serum IGF-1expression of cerebral infarction patients in recovery stage, combined with Danshen injection can further promote serum IGF-1expression highly; But whether separate application of Danshen injection can promote the expression of serum IGF-1still need more large-scale clinical trials further to confirm.2. Both Danshen injection and rhG-CSF can promote the expression of serum VEGF, The application of Danshen injection after rhG-CSF mobilization can further increase the expression of serum VEGF, Danshen injection play a major role in promoting the expression of VEGF.3. Components in Danshen may play its role of inducting stem cells into neuron like cells in vivo through promoting the eppresion of neural repair related cytokine secretion of IGF-1, VEGF.4. After the rhG-CSF mobilization, application of Danshen injection could significantly increase both to promote the recovery of neural function and syndrome of traditional Chinese medicine clinical efficacy. |
