| Objectives To observe the levels of serum VEGF, tumor markers CEA and CA19-9,coagulation indexes and blood platelet count, and discuss the expression pattern of above-mentioned indicators in lung cancer TCM differentiation and especially in blood stasissyndrome.Methods The hospitalized cases meeting the inclusion criteria were divided intosyndromes of blood stasis (BSS), qi deficiency, phlegm damp, phlegm heat and yindeficiency according to the criteria of syndrome differentiation of TCM for lung cancer, inwhich the last four syndromes were identified into the non-BSS group. The serum VEGFlevel was determined using ELISA; the levels of tumor markers CEA and CA19-9weredetected using electrochemiluminescence; the coagulation indexes and blood platelet countlevel were also determined.Results1Comparison of tumor markers in different syndromes: The difference in thelevel of serum tumor marker CEA in these five different TCM syndromes wasstatistically significant (P<0.05), in which the highest serum CEA was detected in theblood stasis syndrome, followed by qi deficiency, yin deficiency, phlegm heat andphlegm damp syndromes. The difference in the level of serum CA19-9in these fivedifferent TCM syndromes was statistically significant (P<0.05), in which the highestresult was detected in the yin deficiency syndrome, followed by phlegm heat, blood stasis,qi deficiency and phlegm damp syndromes.2Comparison of serum VEGF in differentsyndromes: The difference in the level of serum VEGF in these five different TCMsyndromes was statistically significant (P<0.05), in which the highest content wasdetected in the blood stasis syndrome, followed by qi deficiency, yin deficiency, phlegmheat and phlegm damp syndromes.3Comparison of coagulation indexes in differentsyndromes: The differences in the content of plasma APTT, FIB and D-D in these fivedifferent TCM syndromes were statistically significant (P<0.05), while no significantdifference was observed in plasma PT and TT for these five different TCM syndromes,showing no statistical significance (P>0.05). The lowest plasma APTT was detected inthe blood stasis syndrome, followed by qi deficiency, yin deficiency, phlegm heat andphlegm damp syndromes. The highest plasma FIB and D-D was detected in the bloodstasis syndrome, followed by qi deficiency, yin deficiency, phlegm heat and phlegmdamp syndromes.4Comparison of blood platelet count PLT in different syndromes: Thedifference in the level of plasma PLT in these five different TCM syndromes wasstatistically significant (P<0.05), in which the highest plasma PLT was detected in theblood stasis syndrome, followed by qi deficiency, yin deficiency, phlegm heat andphlegm damp syndromes.5Relations of these syndromes with the international TNM-staging: Phlegm damp syndrome dominated in stage I; a higher proportion in stage II fell in qi deficiency, phlegm heat and blood stasis syndromes; the greatest concerns in stageIII were yin deficiency and blood stasis syndromes; qi deficiency and blood stasissyndromes held a higher proportion in stage IV. The difference in TCM syndromes oflung cancer and different clinical stages was significant (P<0.01), and the blood stasissyndrome had a higher proportion in advanced lung cancer.6Comparison of tumormarkers between lung cancer BSS group and non-BSS group: The difference in the levelsof tumor markers serum CEA and CA19-9were statistically significant both in lungcancer BSS group and non-BSS group (P<0.05). Among them, the levels of tumormarkers serum CEA and CA19-9in BSS group were significantly higher than those ofnon-BSS group.7Comparison of serum VEGF between lung cancer BSS group and non-BSS group: The difference in the level of serum VEGF was statistically significant bothin lung cancer BSS group and non-BSS group (P<0.05). Among them, the level of serumVEGF in BSS group was significantly higher than that of non-BSS group.8Comparisonof coagulation indexes between lung cancer BSS group and non-BSS group: Thedifference in the levels of plasma APTT, FIB, D-dimer were statistically significant bothin lung cancer BSS group and non-BSS group (P<0.05), in which the levels of plasmaAPTT, FIB, D-dimer in BSS group were significantly higher than those of non-BSSgroup. The difference in levels of plasma PT and TT were not statistically significant(P>0.05).9Comparison of blood platelet count between lung cancer BSS group and non-BSS group: The difference in the content of PLT was statistically significant both in lungcancer BSS group and non-BSS group (P<0.05), in which the content of PLT in BSSgroup was significantly higher than that of non-BSS group.Conclusions The levels of serum VEGF, CEA and CA199are higher in BSS, which canserve as valuable detection indexes for the diagnosis of lung cancer. The higher levels ofcoagulation indexes APTT, FIB, D-dimer and blood platelet count are observed in the BSS,while the relation of plasma PT and TT with the BSS is not obvious. |
