Protective Effect And Mechanism Of Piperine On Gastric Mucosa In Bile-associated Gastritis Rats

Objective:To investigate the protective effect and mechanism of piperine(the main component of Radix Peperis longi) on gastric mucosa in bile-associated gastritis rats.Methods:The each half of the 60 male and female SD rats were randomly divided into 6 groups:Normal group,model group,high dose of piperine group, middle dose of piperine group, low dose of piperine group and domperidone group. Use normal saline in addition to the normal group, and the remaining groups were gavaged with homemade liquid in order to establish the experimental bile-associated gastritis rats model. After 7 days modeling continuous, then give drug treatment six hours after modeling a day, the experiment lasts 35 days. Serum gastrin (GAS) was determined by radioimmunoassay, measuring C-erbB-2, p21 and PCNA protein expression by immunohistochemistry, RT-PCR method for the determination of gastric mucosal expression of COX-2mRNA content.Results:High, middle and low dose of piperine group of rat with gastric mucosal inflammatory cell infiltration are lesser than the model group (P<0.01).Compared with the model group, the content of rat serum GAS of middle、low dose of piperine group are higher (P<0.05).The P21 expression in rat gastric mucosa of high、middle dose of piperine group and domperidone group are higher than the model group (P<0.05). The high, middle and low dose of piperine group expression of COX-2mRNA are lower than the model group (P<0.01).Conclusion:Piperine which is the main ingredient of Radix Piperis longi can protect the bile reflux gastritis model rat gastric mucosa. The main component of Uygur medicine Radix Piperis longi that piperine can increase serum gastrin、gastric P21 protein content and reduce expression of COX-2mRNA content of bile reflux gastritis rats.