Effects Of 530 Nm Monochromatic Light On BFGF And TGF-β1 Expression In Müller Cells

Purpose: To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor(b FGF) and transforming growth factor-β1(TGF-β1) expression.Methods: Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12, and 24 hour experimental groups, while cells incubated under dark conditions served as the control group. The b FGF and TGF-β1 m RNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.Results: The b FGF m RNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group(P<0.05), while that of TGF-β1 was downregulated(P<0.05). Also, b FGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24 hour group. The changes in b FGF and TGF-β1 fluorescence intensity were highest in the 24 hour group, and significant differences were observed among the experimental groups(P<0.05).Conclusions: The expressions of b FGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 LUX. Müller cells mightplay a role in the development of myopia by increasing b FGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light–induced myopia.