| Objective: To explore guinea pig's refractive state changes, which were reared in different monochromatic lights conditions.Method: To establish four different monochromatic lights houses, each of them has different visual pigment designed to guinea pig maximize absorption of light in different part of the visible spectrum. There are four groups, respectively, green light group (530nm),purple light group (400nm),white light group (dim light) and normal light group (bright light) .There were 40 guinea pigs 3-5 days born, ten of them were reared in each team randomly. Before experiments, the axial length,the vitreous cavity length and refractive states were detected by A scan and retinoscopy. At 2th,4th,6th,8th week, these data were measured again.Result: Result of Refraction: there were almost no difference between dim light group and normal light group (P>0.05); but there were remarkable difference between green light group and dim light group at different time except before experiment (P＜0.01) .the purple light group had no difference within 4 weeks, when compared dim light group, but had remarkable difference at 6th week, at 8th week reached its peak(P<0.01). Result of the length of axial and vitreous cavity: there were remarkable difference in axial length between green light group and dim light group after 4 weeks (P>0.01); and the length of vitreous cavity showed great difference at 2th week (P<0.01) ,and the difference became grater.Conclusion: Monochromatic light may change refractive state stigmatism process of guinea pig; Long wave green light can induce young guinea pig to myopia. Objective To explore guinea pig's retinal photoreceptor cells ultrastructural changes and cone cells differentiation, which were reared in different monochromatic lights conditions.Method To establish four different monochromatic lights houses, each of them had different visual pigment designed to guinea pig maximize absorption of light in different part of the visible spectrum. There were four groups, respectively, green light group (530nm),purple light group (400nm),white light group (dim light) and normal light group (bright light) .There were 32 guinea pigs 3-5 days born, eight of them were reared in each team randomly. To observe the ultrastructural changes of guinea pig's photoreceptor cells by electron microscope ,and research the density changes of guinea pig's cone cell by immunocytochemistry ,8 weeks later.Result Result of electron microscope :Photoreceptor cell outer segments length decreased, membrane discs vacuolization partly, inner segments chondrosome increased, photorecepter cytoblast enlarged, superior retinal half of green group compared to purple group. Photoreceptor cell outer segments length increased, membrane discs integrated, inner segments chondrosome decreased, photorecepter cytoblast shrinked, inferior retinal half of green group compared to purple group. Result of immunocytochemistry: we observed that, in green group, M/L cone cell density increased obviously (P < 0.01) however, in purple group, its density decreased obviously (P < 0.01) ,but there are almost no difference between dim light and normal light group (P > 0.05) .S cone cell dominated in inferior retinal half and its density have no almost difference in four group (P > 0.05) .In green group, photopigment coexpression cone cell density increased obviously (P<0.01) ,but its density decreased in purple group obviously (P < 0.01) ,but there are almost no difference between dim light group and normal light group (P > 0.05) .Conclusion Juvenile guinea pig retinal photoreceptor cells ultrastructure and cone cells differentiation will change during growing in different wave monochromatic lights conditions; in the process of photopigment transformation, green light may contribute to S photopigment converting to M/L photopigment in guinea pig retinal M/L part; photopigment coexpression cone cell may be a kind of cells that will differentiate in certain condition. |
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