Expression Of Basic Fibroblast Growth Factor In The Müller Cell Of Retina In Form Deprivation Myopia Of Guinea Pig

Objective: It was to observe the expression of bFGF in the Müller cell of retina on form deprivation myopia (FDM) in guinea pig to approach the pathogenesis of FDM.Methods: Born 3-4 weeks and weighing 200-250 g matched 50 guinea pigs were divided into five groups, each 10, that is: A: normal group, B: pure cover group, C: to cover group, D: cover + L-a-AAA group [L-a-amino adipic acid (L-a-aminoadipic acid, L-a - AAA)], E: cover + PBS group. FDM model was established on the right eye in guinea pig by shading semitransparent blinders for 2 weeks ; Refractive status and axial length of eyes were determined with retinoscopy after enlargeing pupil and A-scan ultrasonography. Positioning Müller cells and detectioning the expression of bFGF in Müller cells in the retina by GFAP, bFGF immunohistochemistry.Result: 1,Changes of axial length and refraction in FDM eyes.Axial length of FDM eyes growth. Group B (8.40±0.13mm) and group A (7.91±0.11mm) compared to a statistically significant difference (P <0.05); After destroyed the Müller cell, Group D (8.11±0.15mm) and Group E (8.35±0.13 mm) compared to a statistically significant difference (P<0.05).Refraction status of FDM eyes increased, it decreased after removeing blinders. Group B (-1.70±0.16D) compared with Group A (+1.55±0.29D) and group C (-0.65±0.10D) to a statistically significant difference (P <0.05); After destroyed the Müller cell, Group D (-0.85±0.13D) and Group E (-1.70±0.21D) compared to a statistically significant difference (P <0.05).2,Identification of the Müller cells of the retinaThe results of GFAP staining showed GFAP were mainly expressed in the cytoplasm of Müller cells in the outer nuclear layer , the outer plexiform layer and the inner nuclear layer of the retina. Irregular shape of the nucleus of Müller cells , with spindle-shaped, oval, etc ,located at the inner nuclear layer of the retina.Processes of Müller cell through outside membrane and inside membrane likes grating in retina.3,Expression of bFGF in the Müller cells of retina.bFGF expressed in Müller cells of each group in the retina ,maining in processes in the inner plexiform layer. Semi-quantitative analysis of immunohistochemistry slice showed that the positive value of gray area in group B (136.35±13.52) and Group A (104.62±11.85), C group (117.83±14.25) compared to a statistically significant difference (P <0.05). After destroyed the Müller cell, Group D (150.57±12.05) and Group E (137.47±12.95) compared to a statistically significant difference (P <0.05).Conclusion: 1 Müller cells in the retinal may be involved in the formation of FDM.2 Expression of bFGF in the Müller cells of retina may be related to FDM.