| Objective:To observe the effects of Huoxue Jiangzhi Baogan decoction(HJ BD)healed on non-alcoholic fatty liver disease(NAFLD) rats, and to explore the role of HJBD decoction in the progress of NAFLD healing. Methods:60 healthy male SD rats were randomly divided into normal group, model group, high-dose HJBD group, mid-dose HJBD group, low-dose HJBD group and group of QiangGan-Pian. Besides normal group which was raised by standard animal diet, the others were given high fat diet.All rats were sacrificed at the end of the 12 th week,collected and tested the Serum samples of the liver function(AST, ALT)and blood lipid(TG,TC).Morphological changes of Liver tissue were obs erved under microscopy by HE stain, the expressions of Nrf2 and PKB in liver tissue were assayed by immunohistochemical(IHC).Results:Compared with normal group, the liver function(AST, ALT)and blood lipid(TG,TC)of model group were significantly increased,the differ- ence was statistically significant( P <0.01)。Compared with model group,the liver function(AST, ALT)and blood lipid(TG,TC)of HJBD and Qiang-Gan-Pian groups were significant decreased,the difference was statistically significant( P < 0.01)。 immunochistochemical staining showed that Compared with normal group, Nrf2 expression in model group was increased and PKB expression in model group was decreased, but the difference were not statistically significant。 Compared with model group, Nrf2 and PKB expression in HJBD and Qiang-Gan-Pian groups were significant increased, the difference was statistically signif- icant(P < 0.01),but the increase of HJBD of high-dose group was more obvious. under microscopy by HE stain showed that HJBD can significant- ly decrease hepatocytes swelling, lipid deposition and inflammatory cells infiltration in the liver tissues.Conclusion:HJBD can signifycantly decrease NAFLD level of hepatocytes lipid deposition and regulate lipid metabolism, improve blood biochemical indices and liver function, at the same time it can increase the Nrf2 and PKB expression in liver tissue 。 These was One of the possible mechanism of prevention of NAFLD. |
