| Pathophysiology important reason excessive apoptosis of myocardial ischemia-reperfusion injury occurs during myocardial cells is once again leading to myocardial injury, myocardial apoptosis as ischemia-reperfusion injury in the early events throughout the ischemia-reperfusion injury process. Myocardial damage is caused by many factors ischemic tissue metabolic dysfunction and structural damage, so that the extent of tissue damage further.Purpose: Preliminary discussion yanhusuo and borneol drugs on protection of myocardial injury in mice and its mechanismMethod: The use of isoproterenol(25mg / kg) caused C57 BL / 6 acute myocardial injury model approach, C57 BL / 6 mice were randomly divided into 7 groups 105 blank control group, model group, the test drug group(1-50 mg ? kg-1 ? d-1), n = 15. Previously orally administered drug for borneol fumarate(1-50 mg ? kg-1 ? d-1) 7 days after continuous administration ISO(25 mg ? kg-1 ? d-1) 7 天 cardiac hypertrophy model was prepared. Serum enzymes CAT, NO, SOD, MDA content in the observed fumarate- borneol drugs on myocardial injury induced by ISO model and a dose-dependent inhibition. Comparison of myocardial mass and body weight in mice, observe the effects of drugs on acute myocardial injury in mice pathological morphology.Result: Corydalis- borneol drug dose-dependent increase in serum levels of CAT, with a significant(P <0.05) difference compared with the model group; each dose group were seen in serum NO content increased, with the model group, the difference was significant significance(P <0.05); Corydalis borneol Drugs may also be dose-dependent increase in serum SOD activity, with the model group and the difference was significant(P <0.05); MDA levels in Corydalis borneol drugs on different dose groups were seen significantly reduced, with a significant(P <0.05) difference compared with the model group; each group of mice to directly measure body weight(BW) and heart weight(HW), and for(HW / BW) ratio. Compared with the saline group, a significant increase in the mice injected HW ISO’s. After applying CYH can significantly reduce the HW and HW / BW; borneol drugs on pre-application Corydalis(Corydalis yanhusuo, CYH)(25 mg·kg-1·d-1) can significantly reduce the degree of myocardial hypertrophy in mice. Gross observation of cardiac status change appearance, mainly in the heart of the volume increases, conventional cardiac tissue sections stained with hematoxylin-eosin(HE staining). Myocardial tissue sections stained with HE. Compared with the saline group, ISO treated mice CM large cross section, CYH can suppress an increase in cross-sectional area of the myocardium in mice caused by ISO.Conclusion: In this experiment, after the ISO-induced myocardial ischemia, serum CAT and SOD content decreased, increased MDA content; fumarate borneol drug can increase serum of CAT and SOD content in each dose group were lower levels of serum MDA. It follows that for fumarate borneol medicine after myocardial ischemia showed significant anti-peroxidation.NO has strong diastolic blood vessels, anti-platelet aggregation and inhibition of neutrophil aggregation and adhesion. The results found, yanhusuo borneol can increase the NO content in the blood, presumably it after myocardial ischemia has certain diastolic blood vessels, promote the blood supply and anti-inflammatory effect.HE staining results showed that the application medicine yanhusuo borneol in advance(Corydalis yanhusuo, CYH)(25 mg·kg-1·d-1) can obviously reduce the degree of myocardial hypertrophy in mice. HE staining on myocardial tissue biopsies. Compared with saline group, ISO treatment group mice CM cross-sectional area is larger, CYH can inhibit the ISO of mice caused by myocardial cross sectional area increase. |
PDF Full Text Request