Study The Material Basis Of Corydalis Rhizoma For The Treatment Of Myocardial Ischemia

Objective To study the material basis of Corydalis for the treatment of myocardial ischemia and its possible mechanism.Method The traditional Chinese medicine fingerprint technique,molecular docking technology and network pharmacology method were used to study the material basis and possible mechanism of the treatment of myocardial ischemia.Fingerprint analysis of 10 batches of Corydalis medicinal materials was carried out by HPLC method and using the chromatographic fingerprint similarity evaluation system of Chinese medicine.The column was Diamonsil Plus C18(250×4.6mm,5?m),the column temperature was 30°C,and the mobile phase was Acetonitrile-0.2% aqueous acetic acid solution,gradient elution,flow rate of 1.0 ml/min,detection wavelength of 280 nm;male rats were selected into 7 groups,8 rats in each group,divided into sham operation group,model group,isosorbide mononitrate Ester group,tertiary amine base low concentration group 10mg/kg,tertiary amine base high concentration 40mg/kg,quaternary amine low concentration group 10mg/kg,quaternary ammonium high concentration group 40mg/kg,continuous preventive administration for 7 days,the first Half an hour after 7 days of administration,acute myocardial ischemia was induced in rats by coronary artery ligation.Then,the electrocardiogram of each group was detected.The rat heart tissue sections were taken and stained by TTC to observe the ischemia status of each group.Serum biochemical parameters were measured after centrifugation of abdominal aorta,and serum endothelin(ET),nitric oxide(NO),nitric oxide synthase(eNOS),thromboxane(TXB2),prostacyclin(PGI2),Changes in enzymes related to myocardial injury,such as lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),and aspartate aminotransferase(AST),observed myocardial injury in rats,and studied in Corydalis Therapeutic effects of two types of alkaloids on acute myocardial ischemia in rats;combined with TCMSP platform to screen the target small molecule compounds,and using Maesrto11.1 molecular docking software to screen small molecules with good target protein in Corydalis Compounds,then use Cytoscape 3.6.1 to construct a multi-component-target network pharmacology map,and explain its network characteristics through topology analysis.Results The 14 common peaks were determined,and the fingerprints of the Rhizoma Corydalis were established.The similarity of the fingerprints of 10 batches of Corydalis medicinal materials was more than 0.9,and the contents of 9 alkaloids were determined.Compared with the model group,the high dose of quaternary amines The electrocardiogram of myocardial ischemia in the treatment group showed that the ST segment was significantly decreased.The different doses of quaternary amine treatment could reduce the infarct rate of myocardial cells.The different doses of quaternary amine treatment could decrease the creatine kinase isoenzyme CK-MB and lactate.Hydrogenase LDH,aspartate aminotransferase AST level;quaternary amine high-dose treatment group can increase NO content,eNOS activity value,quaternary amine base treatment group can reduce endothelin ET content;quaternary amine base different doses The treatment group was able to increase the prostacyclin PGI2 level,and the high-dose quaternary amine group reduced the thromboxane TXB2 level;in the 16 myocardial ischemic target docking results,8 quaternary amine compounds,1 flavonoid,2 tertiary Amine bases show good docking results.The docking results of quaternary amine compounds and flavonoid quercetin were generally better than those of tertiary amine bases.In the docking results,there were 10 target proteins with higher average quaternary amine base scores than tertiary amine bases.The results of network pharmacology analysis showed that the network heterogeneity of multi-compound-target was 0.57,the average number of adjacent nodes was 3.59,the characteristic network length was 3.02,and the network centrality was 0.21.Conclusion Corydalis quaternary amine compound coptisine,palmatine,dehydrocorybulbine,jatrorrhizine,columbamine,berberine,flavonoid quercetin may be the material basis for the treatment of myocardial ischemia,Corydalis treatment of myocardium Ischemia is the result of multiple components interacting with multiple targets.Quaternary amine base and flavonoid quercetin may inhibit the cardiomyocyte energy metabolism,reduce myocardial cell apoptosis,inhibit the cardiomyocyte "injury-enzyme release-re-injury" vicious circle,clear cardiomyocyte oxygen free radicals,reduce cardiomyocytes Inflammatory response,changes in blood rheology to reduce blood viscosity,in order to achieve anti-ischemic effects.