| ObjectiveDiscuss sini decoction for isopropyl adrenaline causes the protective effects of myocardial fibrosis in rats and its mechanism of action.MethodsThirty-six Wistar rats were randomly divided into control group, model group, and Sini decoction group, 12 rats in each group, all rats were adaptability fed for 1 week.The models were established by subcutaneous injection of isoproterenol(5 mg/kg·d) for 8 days. From the ninth day,rats were treated with Sini decoction 6.06g/(kg·d) by intragastric administration for 4 weeks. Heart weight index(heart weight/body weight,HW/BW) were measured after the experiment was finished, the contents of hydroxyproline(Hyp) was assayed with spectrophotometry respectively, the collagen volume fraction(CVF) of myocardial interstitial was calculated by Masson staining image analysis, the level of intercellular cell adhesion molecule-1(ICAM-1), transforming growth factor-β1(TGF-β1) in plasma were determined with the ELISA, the protein expressions of PPARα and PPARγ were detected with Western blotting. Sini decoction group ratsResults1. Cardiac weight index results: compared with control group, model group of cardiac weight index(HWI) and left ventricular weight index(LVWI) increased significantly(P< 0.05); compared with model group, the cardiac weight index(HWI) and left ventricular weight index(LVWI) decreased significantly in Sini decoction group(P< 0.05); the difference had statistic significance.2. Masson staining results: the collagen fibers were blue, myocardial cell and muscle fibers were red. We can see under a microscope: myocardial fibers of control group were in order, transverse striation arranged obviously, the nucleus was clearly visible; myocardial fibers in model group arranged not neat, transverse striation blurred, individual cells appeard focal or patchy necrosis, there was a large number of inflammatory infiltration in interstitial, myocardial fiber cell hyperplasia four decoction group was obviously less than model group, the degree of myocardial fibrosis have improved significantly. The amount of collagen fiber four decoction group was obviously less than model group, there are still a small amount of increased compared with control group. Through calculation and analysis, model group of CVF is higher than the control group(P < 0.05), four decoction group of CVF is lower than the model group(P < 0.05), the difference was statistically significant The results of AngⅡ concentration in serum: AngⅡvalues of model group were higher than sham operation group(P<0.05), and compared with model group, Ang II values significantly decreased in all treatment groups(P<0.05), the difference had statistic significance. There was no significant difference between Captopril group and Sini Decoction group(P>0.05).3. Hydroxyproline content of myocardial tissue in all groups of rats: hydroxyproline content in myocardial tissue of model group rats was significantly higher than control group(P<0.05), hydroxyproline content in myocardial tissue of Sini decoction group significantly lower than model group(P<0.05).4. ICAM-1and TGF-β1 level in plasma of all groups rats: ICAM-1and TGF-β1 level in plasma were detected by ELISA method, according to the results after treatment, compared with control group, ICAM-1and TGF-β1 level in plasma of model group rats increased significantly, while compared with model group, ICAM-1and TGF-β1 level in Sini decoction group were significantly reduced, the differences had statistic significance(P<0.05).5. Immunohistochemistry and western blot results: compared with control group, PPARα and PPARγ protein expression in model group decreased, compared with model group, PPARα and PPARγ protein expression in Sini decoction group increased, so Sini decoction can effectively up-regulate PPARα and PPARγ protein expression in myocardial tissue.ConclusionsSini decoction can alleviates myocardial fibrosis induced by isoproterenol, and its mechanism may be related to increasing the expression of PPARα and PPARγ protein in myocardium, decreasing the level of ICAM-1 and TGF-β1. |
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