The Research Of The Protection Of Yanhusuo Total Alkaloids On Acut Myocardial Ischemia Rats Model Of Iso Induction

Objective: To observe the protection of different doses of yanhusuo total alkaloids on acut myocardial ischemia model rats of ISO induction, and explore possible mechanism, it may provide experiment evidence for treating clinical ischemia cardiovascular disease safely and rationally by yanhusuo total alkaloids, and at the same time it also lays the foundation for further studying owning proprietary intellectual property rights of preventing myocardial ischemia of original new drugs of yanhusuo types.Method: To make acut myocardial ischemia model of rats by injecting Iso according to Rona, recording ECG II and left ventricle pressure by BL-420F organism function experiment system, assaying serum myocardial enzyme and content of MDA, SOD in serum by 722 model visible-infrared spectrometer and assaying water content in myocardial tissue, they act as observation indicators. Firstly, detecting ECG II of health and adult Wister rats (weight220±20g, use both female and male, purchase from laboratory animal centre of Heilongjiang university of traditional Chinese medicine), to reject those objects whose ECG is abnormal, and those whose ECG is normal are random divided 6 groups: normal group, model group, yanhusuo total alkaloids high doses group, yanhusuo total alkaloids middle doses group, yanhusuo total alkaloids low doses group, positive control group, 10 objects per group. Yanhusuo total alkaloids low doses, middle doses and high doses group, which are respectively given 0.5mg/kg, 1.0mg/kg, 2.0mg/kg yanhusuo total alkaloids lavaged per day, partly laveged one time morning and evening. Normal group and model group are lavaged the same volume distilled water. Positive control group was given Kedaling, according to 0.5 mg/kg lavaged lasting for seven days. After one hour each group rats were lavaged for the last time, to anesthesia by injecting 20% carbamate 5ml/kg from abdomen, fix on iron support by overhead position, cut fur on the neck, disinfect in iodine, deiodination in alcohol, separate right carotid artery by asepsis surgen after laying gauze with a hole, join PT-100 organism blood pressure sensor and insert full of heparin(2mg/100ml)with diameter of (0.5mm) polytene cardiac catheter to left ventricle to detect the change curve of left ventricle pressure, and apply electrode prick into limbs subcutaneous of rats to record ECG II varying curve synchronization, after ECG and ventricle pressure stable, except normal group rats, other groups multi-point hypodermic inject in ISO(6mg/kg), to record ECG II and left ventricle pressure curve of rats by BL-420F organism function experiment system after injection iso synchronization, analyze the change of HR, ST, T wave, LVSP, LVEDP, +dp/dtmax, -dp/dtmax, on the 5s, 10s, 5min, 10min, 30min, 60min, 120min, 180min until 360min, take blood 2ml from left carotid artery, centrifugate blood serum in the condition of 4℃. Examine the change of LDH, CK, CK-MB, SOD, MDA in blood serum by 722 model visible-infrared spectrometer, after obtain blood, scissors cavitas and get heart at once, wash it with ice salt water and remove the big vessel and bindweb, weigh wet weight of the hearts by electronic balance, and then bake 24h in constant temperature drying baker in 85℃, and then take out when it is constant weight, weigh dry weight, by (wet weight minus dry weight )/ wet weight×100%, examine the change of water content of myocardiac issue.Normal group rats don't inject iso, only observe in corresponding time and make normal contrast.Results:1,Model group rats HR in ECG increase evidently, ST descend distinctly, T wave range ascent, exist significant difference in contrast with normal group by statistics; Model group LVSP decrease evidently, LVEDP increase distinctly,±dp/dtmax slow, exist significant difference in contrast with normal group; Model group LDH, CK, CK-MB evidently step up and MDA increase, SOD decrease, exist significant difference in contrast with normal group; Model group water content of myocardiac issue apparently heighten, exist significant difference in contrast with normal group.2,Yanhusuo total alkaloids high doses group, middle doses group HR decrease, ST ascend, T wave range descent, exist significant difference in contrast with model group by statistics, low doses group the change of HR, ST, T wave without significant difference in contrast with model group; Yanhusuo total alkaloids high doses group, middle doses group LVSP increase, LVEDP decrease,±dp/dtmax accelerate, exist significant difference in contrast with model group, low doses group LVSP, LVEDP,±dp/dtmax, without significant difference in contrast with model group; Yanhusuo total alkaloids high doses group, middle doses group LDH, CK, CK-MB step down, MDA decrease, SOD increase, exist significant difference in contrast with model group, low doses group LDH, CK, CK-MB, MDA, SOD, without significant difference in contrast with model group; Yanhusuo total alkaloids high doses group, middle doses group water content of myocardiac issue apparently reduce, exist significant difference in contrast with model group, low doses group water content of myocardiac issue, without significant difference in contrast with model group.3,Positive control group HR decrease, ST ascend, T wave range descent, LVSP increase, +dp/dtmax accelerate, LDH, CK, CK-MB step down, MDA decrease, SOD increase, the water content of myocardiac issue reduce, exist significant difference in contrast with model group.4,Yanhusuo total alkaloids high doses group the change of LVEDP, -dp/dtmax, exist significant difference in contrast with positive control group.Conclusion:1,This experiment succeed to duplicate acut myocardial ischemia rats model by multi-point hypodermic injecting in ISO(6mg/kg).2,Yanhusuo total alkaloids high doses group(2.0mg/kg), middle doses group(1.0mg/kg) have protect function to the rats model of acute myocardiac ischemia that is tempted by continous injecting ISO, and promote restoring of heart pumping blood.3,This protect function may be connected with the following mechanism:①Yanhusuo total alkaloids can improve the activity of endogenous OFR enzyme system, inhibit the reaction of myocardium lipo-overoxidation, and lessen myocardiac cell impairment because of too much OFR;②Protect myocardiac cell membrane, keep stabilization of myocardiac cell membrane, reduce leaking of myocardiac enzyme;③Improve LVSP, LVEDP and±dp/dtmax, promote restoring of heart function.