| Preeclampsia is unique complications of pregnancy patients, threatening the health of maternal and child. The incidence rate is about 5%-12%, for primipara, the incidence rate is approximately 3%-7%, while for maternal, the incidence rate is approximately 3%-7%. Researches show that variety of factors including vascular endothelial damage, insulin resistance, placenta shallow implantation, oxidative stress, and placental ischemia can led the occuring of preeclampsia. The main pathophysiology change of this disease consistently thinked was spasm of systemic small vessel and endothelial damage and local ischemia. Research data shows that inflammatory response, insulin resistance (insulin resistance, IR), and lipid-lowering effect of fat cells of vascular endothelial cells are related with disorder of endothelial function. Resistin, Adiponectin, and Apelin which secreted by fat cells are related with the inflammation of vascular endothelial and insulin resistance. The insulin resistance and endothelial damage all promote or increase the development of hypertensive disorders in pregnancy. Apelin as an inflammatory factor participats the process of body inflammation and cell growth and apoptosis. Adiponectin by activating TNF-α, NF-κB pathway direct response to inflammatory cells play an anti-inflammatory effect. Resistin may activate phospholipase C and P38MAPK involved in the inflammatory process.Objective:Apelin, adiponectin and resistin expression in the case of pre-eclampsia patients were detected and the relationship between the three diseases indicators with pregnancy-induced hypertension was analyzed. The effect and mechanism of three diseases indicators in early eclampsia was investigate.Methods:1 Research subjects:(1) experimental groups:60 cases of hypertensive disorders in pregnancy patients in our hospital (Cangzhou City People’s Hospital) from March 2013 to February 2014 were randomly selected. The patients were including mild preeclampsia patients and patients with severe preeclampsia (each 30 cases). (2) control group:30 cases of healthy pregnant people in our hospital in the same period were selected. There were no patients with diabetes, chronic hypertension, chronic liver disease, kidney disease, immune system disorders, or wwin pregnancy history in both groups.2Experimental method:4 ml fasting cubits venous blood of three groups were collected in morning. The sample were centrifuged for 10 minutes by2000 r/min and were saved in -70℃ refrigerator. The serum apelin, adiponectin and resistin of three groups were detected by enzyme-linked immuno sorbent assay.3 Statistical analysis:All data we get were analyzed by SPSS 13.0.① measurement data was analyzed by ANOVA.② Comparison between two groups were analyzed by T-test.③Correlation analysis between the two variables were analyzed by Spearman rank correlation analysis. P=0.05 was the significantly different standards of each group.Results:1 Apelin level of severe preeclampsia group was significantly higher than light preeclampsia group and control group. The apelin level of three groups were 92.40±7.99,68.56±6.29, and 51.21±6.01 ng/L. The differences were statistically significant.2 The adiponectin level f severe preeclampsia group was significantly higher than light preeclampsia group and control group. The adiponectin level of three groups were 4.61±2.12,7.12±2.65, and 12.14±3.56pg/ml. The differences were statistically significant.3.The resistin level f severe preeclampsia group was significantly higher than light preeclampsia group and control group. The resistin level of three groups were 28.75±5.01,21.69±5.12,14.02±4.41 ug/L. The differences were statistically significant.4 The serum apelin levels was negatively correlate with the serum adiponectin levels(r=-0.597, P<0.01; r=-0.786, P<0.01; r=-0.636, P<0.01). The serum resistin levels was negatively correlate with the serum adiponectin levels (r=-0.764, P<0.01; r=-0.528,P<0.05; r=-0.540, P<0.01)Conclusion:1 In our research, apelin, adiponectin, and resistin all have expression in three groups, and with the increase of preeclampsia the expression of apelin and resistin were higher and adiponectin was lower..2 For preeclampsia patients, apelin and resistin were negatively correlated with adiponectin. Three indicators were interactions and inseparable, effect the occurrence and development of preeclampsia. |
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