Application Of Aescuven Forte To Alleviate The Postoperative Inflammation Of Tension-free Herniorrhaphy

Objective Using rabbits as experimental object, muscle-thickness abdominal wall defect models were established, with the method of packet control, to study the postoperative inflammation of tension-free herniorrhaphy, and the application of Aescuven forte to alleviate the postoperative inflammation of tension-free herniorrhaphy.Objective Using rabbits as experimental object, muscle-thickness abdominal wall defect models were established, with the method of packet control, to study the postoperative inflammation of tension-free herniorrhaphy, and the application of Aescuven forte to alleviate the postoperative inflammation of tension-free herniorrhaphy.Methods Randomly selected 18 rabbits of clean grade, from the animal laboratory of Anhui medical university, muscle-thickness abdominal wall defect models were established, and randomly assigned to one of three groups:repaired without any mesh(negative control group, A), repaired by polypropylene mesh group(control group, B), and the group was repaired by polypropylene mesh after oral Aescuven forte(experimental group, C):2 days before the operation for medicine, daily 100mg/kg. Acquisition biood from ear vein of the rabbit at the time points of preoperative and after 1,4,7,20 d, Centrifugal serum, and the expression of tumor necrosis factor(TNF)-a and interleukin(IL)-β was detected simultaneously by using radioimmunoassay(RIA) and on the postoperative day 1,4,7 and 20 in each group. Using the variance analysis to compare within group, and the q test for comparison between groups; kill the rabbits with the air needle after 20d, observe and score the incision tissue edema,using poncirus related inspection to analysis swelling scores; take the surrounding tissue adhesion after the rabbits were killed, and immersed into 10%Formaldehyde Solution, according to conventional methods for specimens for HE staining, and observe the pathological change with light microscope.Results There was no death in 3 groups, abdominal incision infection occurred in 1 cases in B group. The score of incision edema of the rabbits in the experience group(n=6) was 3,and the control group(n=6) was 13,the difference was statistically significant between the 2 groups(P<0.05);The preoperative differences of the levels of TNF-a and IL-1β in 3 groups showed no significance[TNF-α was (1.35±0.14)、 (1.19±0.12)、(1.11±0.32)μg/L respectively and IL-1β was (0.052±0.011). (0.053±0.022)、(0.073±0.024)μg/L respectively, P>0.05)];4 and 7 days after the operation, the levels of TNF-a and IL-1β in the control group were significantly increased as compared with the blank group and the experience group[control group TNF-awas (3.40±0.59)μg/L and(5.5±0.8)μg/L,IL-1βwas(0.35±0.161)μg/L and(0.655±0.132) μg/L, blank group TNF-αwas (1.48±0.29) μg/L and (1.6±0.4) μg/L, IL-1β was (0.113±0.015) μg/L and (0.120±0.015) μg/L, experience group TNF-α was (2.07±0.53) μg/L and (2.4±0.6) μg/L, IL-1β was (0.237±0.079) μg/L and (0.237±0.079) μg/L, P<0.05]. Compared with the blank group, inflammatory cytokines were significantly increased in experimental group and control group, the biggest difference was at 1 weeks after operation, however, the increased levels of experimental group was significantly reduced than the control group, it was showed that there is still a certain inflammatory response after taking Aescuven forte, Aescuven forte can alleviate the postoperative inflammation of tension-free herniorrhaphy and worthy of popularization and application.Results There was no death in 3 groups, abdominal incision infection occurred in 1 cases in B group. The score of incision edema of the rabbits in the experience group(n=6) was 3,and the control group(n=6) was 13,the difference was statistically significant between the 2 groups(P<0.05);The preoperative differences of the levels of TNF-a and IL-1β in 3 groups showed no significance[TNF-α was (1.35±0.14)、 (1.19±0.12)、(1.11±0.32)μg/L respectively and IL-1β was (0.052±0.011). (0.053±0.022)、(0.073±0.024)μg/L respectively, P>0.05)];4 and 7 days after the operation, the levels of TNF-a and IL-1β in the control group were significantly increased as compared with the blank group and the experience group[control group TNF-awas (3.40±0.59)μg/L and(5.5±0.8)μg/L,IL-1βwas(0.35±0.161)μg/L and(0.655±0.132) μg/L, blank group TNF-αwas (1.48±0.29) μg/L and (1.6±0.4) μg/L, IL-1β was (0.113±0.015) μg/L and (0.120±0.015) μg/L, experience group TNF-α was (2.07±0.53) μg/L and (2.4±0.6) μg/L, IL-1β was (0.237±0.079) μg/L and (0.237±0.079) μg/L, P<0.05]. Compared with the blank group, inflammatory cytokines were significantly increased in experimental group and control group, the biggest difference was at 1 weeks after operation, however, the increased levels of experimental group was significantly reduced than the control group, it was showed that there is still a certain inflammatory response after taking Aescuven forte, Aescuven forte can alleviate the postoperative inflammation of tension-free herniorrhaphy and worthy of popularization and application.Conclusion The swelling degree of incision area was obviously reduced by taking Aescuven forte, Aescuven forte can effectively reduced the expression of cytokines after operation, so as to alleviate the systemic cellular immune response. So Aescuven forte can use in clinical application, and effectively reduce the postoperative complications caused by inflammatory reaction after the tension-free herniorrhaphy.