A Study On Oral Microbial Community Structure Of Periodontitis Between Dongxiang And Yugurs In Gansu Drovince

Objective:Chronic periodontitis were selected from Dongxiang and Yugur in Gansu province.By constructing 16S rRNA clone libraries to compared the microbial community structure differences from different taxonomic levels between the two groups. To explore two main periodontal pathogens of these two groups, and provide theoretical support for further study of microbial pathogenesis. And take early preventive measures from the perspective of the etiology, to reduce the incidence of periodontitis and promote periodontal health.Methods:According to the WHO sampling standards,20 saliva samples collected from two group periodontitis patients, labeled:DP (Dongxiang periodontitis samples), YP (Yugurs periodontitis samples). Bacterial genomic DNA were extracted, fragments were amplified by PCR and then transformed into E.coli DH5a to build 16SrRNA clone library.16SrRNA were sequenced, eliminate unqualified sequence. Finally, softwares and related databases such as MOTHUR, RDP, NCBI and MEGA were used to analysis and get microbial taxonomyinformations.Results:(1) 129 OTUs were detected from saliva samples of these two groups, attributed to the 7phyla and 37 genera.(2)7 bacterial phyla:Firmicute, Proteobacteria, Actinobacteria, Bacteroidetes, Fusobacteria, Synergistetes and TM7. Wilcoxon test for the abundance of 5 phyla showed that no statistical significance (P> 0.05) between two groups.(3) The dominant bacteria detected from two groups samples existed differences:DP group were Streptococcus (36.81%), Neisseria (10.44%), Gemella (5.49%), Prevotella (4.95%), Veillonella (3.85%), Actinobacillus (3.30%), Porphyromonas (2.75%), Fusobacterium (2.20%), Haemophilus (2.20%), Granulicatella (1.10%), Leptotrichial (10%), Peptostreptococcus (1.10%) and TM7 (1.10%).YP group, were Streptococcus (43.7%), Methyloversatilis (7.10%), Pseudomonas (5.20%), Acinetoact (4.40%), Schlegelella (4.00%), Neisseria (3.60%), Gemella (3.60%), Prevotella (3.20%), Haemophilus (1.60%), Eubacterium (1.60%) and Fusobacterium (1.20%).(4) Streptococcus presented in all 20 samples, considered as the core microorganism in saliva of these patients with periodontitis;Besides, the foregone species has been classified, but there are 11 species which can not accurate classification, so the role of the occurrence and development of these bacterial genus in clinical periodontitis will need to further study.(5) Distributions of Aggregatibacter (DP group:0.00%, YP group:3.30%), Neisseria (DP group:10.40%, YP group:3.60%) and Veillonella (DP group:0.40%, YP group:3.85%) in two groups samples having a significant difference (p<0.05).Conclusion:16S rRNA clone library method can be used to study the oral microbial community structure in patients with periodontitis.There are some differences of oral microbial community structurebetween Dongxiang and Yugur periodontitis in Gansu province. Differenceson Aggregatibacter, Neisseria and Veillonella between the two ethnic patients with periodontitis need ain-depth study. The role of Streptococcus, Prevotella, Fusobacterium, Haemophilusand other common oral periodontal pathogens in periodontitis patients deserves further study.