Study On The Differences Of Oral Microbes In Different Populations And The Improvement Of Oral Environment

The oral micro-ecosystem is composed of a large number of microorganisms,which is closely related to the health and diseases of the whole body,and is easily affected by lifestyle habits,dietary rules,tobacco products,etc.,which in turn affects human health.Current scientific research shows that smoke or oral products have an impact on a variety of microorganisms in the oral cavity,but most of the data are based on epidemiological investigations or in vitro culture experiments of oral microorganisms,which have great limitations.The overall effect of oral microbial effects and the relationship between this effect and oral health is still unknown.Based on metagenomics technology,this project designs a scientific and reasonable population cohort sampling plan.Through large-sample 16S rRNA sequencing,the oral microbes of different populations(smokers and non-smokers)are studied in depth and systematically from the perspective of oral microbes.The scientific understanding of microbes is role in oral health and oral diseases,objectively assess the impact of different lifestyle habits on oral microbes.And through in vitro antibacterial experiments,the development of natural extracts of medicinal and edible plants that can improve the oral environment provides a new basis for oral health.Objective:Analyze the differences in oral microbes of different populations,evaluate the impact of different habits on the oral environment,and explore natural extracts of medicinal and edible plants that inhibit oral pathogenic microorganisms..Method:First,through 16S rRNA sequencing,bioinformatics analysis of saliva samples from smokers and non-smokers was performed to compare the differences in oral microbial diversity between the two;secondly,the characteristics of smoking years,number of cigarettes,nicotine dependence,and oral ulcers were compared.Carry out specific analysis on the influence of oral microbial composition to explore the diversity of oral microbes in different habitual populations;then verify in vitro studies and use traditional isolation methods to separate and identify oral microbes from different populations,and compare the sequencing results to obtain common strains;finally,in vitro antibacterial Experiments to screen natural extracts of medicinal and edible plants(Prunella vulgaris L,Prunus mume(Sieb.)Sieb.etZuce,Sappan Lignum,Arctium lappa L,Gardenia jasminoides Ellis,Rhus chinensis Mill,Granati Pericarpium)that have inhibitory effects on some oral pathogens.Results:There are significant differences in the oral microbial diversity of different populations.Actinobacteria are enriched in smokers.The species under the phylum invade the inside of the tissue when the cheeks,gums and other parts are damaged,which can easily cause Actinomycosis(Tuberculosis,Leprosy);smoking years and the number of smoking have a significant impact on the composition of oral microbes.With the increase of smoking years and number of smoking,the diversity of oral microbes gradually increases,which affects oral health;whether different nicotine dependence and oral ulcers are easy sensation has no significant effect on the composition of oral microbes.The natural extracts of medicinal and food homologous plants have inhibitory effects on some oral pathogens,and the antibacterial effect of gardenia extract is stronger than that of other medicinal and food homologous plant extracts.Conclusion:There are significant differences in oral microbial diversity in different populations.Smokers have higher species diversity than non-smokers.The effect of tobacco on oral microbes may be a gradual process.Both smoking years and number of smoking can affect oral microbes These changes mean that the smoker’s oral environment may have changed.Natural extracts of medicinal and edible plants have inhibitory effects on certain strains in the oral cavity,and can be applied to the research and development of substances that improve the oral environment.