The Expression Of NGAL In The Blood, Urine And Placenta Tissue Of Patients With Hypertension Of Pregnancy And Preeclampsia

Purpose: Hypertion disorder complicating pregnancy(HDP) is a major disease which happens with pregnancy and blood pressure rising, including pregnancy-induced hypertension, preeclampsia, eclampsia gravidarum as well as chronic hypertension preeclampsia and chronic hypertension complicating pregnancy. The pathogenesis of HDP is not yet clear. Researchers have generally agreed that the occurrence of HDP is lead to a kind of factors, but the central pathogenesis is the activation and injury of maternal systemic disputation endothelial cells. The mechanism of endothelial activation and injury is unknown. Therefore, we assume that if there is a way to assess the endothelial damage and determine the extent and development of disease, it is possible to give a reasonable prevention and treatment which is great significance to the clinical diagnosis and treatments of HDP.Neutrophil gelatinise-associated lipocalin(NGAL) is a newly discovered lipoprotein which can promote the growth and differentiation of many cells and proteolytic and degradation of collagen in organization. Studies have pointed out that NGAL is the maker which can relate to the level of endothelial injury cansed by inflammation and tumor happened. The lever of NGAL had been elevated when the inflammation and tumor happened. Numerous studies confirm that NGAL is an early indicators of renal failure and acute inflammation, with a high degree of sensitivity and specificity. Furthermore, a related study found that the level of serum NGAL in the patients with hypertension is significant increase than the normal group. For early-onset preeclampsia and late-onset preeclampsia, the expression of NGAL in blood, urine and placental tissue were still unknownThe aim of our study is to explore the expression of NGAL in blood, urine and placental tissue of patients with early preeclampsia and late preeclampsia. By comparing the level of normal person to find the function of NGAL in the occurrence and development of preeclampsia, and provide theoretical basis for the research on the pathogenesis of preeclampsia.Method:1 Specimen collectionThe patients are selected from the hospitalization of pregnant women in Han Dan No.2 Hospital from December in 2013 to November in 2014, including 20 cases of early-onset preeclampsia, 20 cases of late-onset preeclampsia and 20 cases of normal pregnant women as control group. All the pregnant women are expected for singleton pregnancy, without history of recurrent spontaneous abortion and assistant reproductive technology. Gestational diabetes mellitus, gestational hypertension, gestational cardiac disease and other medical and surgery complications are excluded.2 Use ELISA assay to detect the NGAL level in blood and urine of pregnant womenThe Specimen are divided into 6 groups. Group A: pregnant women with early-onset preeclampsia(EOPE), age(20-35), gestational weeks(20 weeks-34 weeks, including 20 weeks). Group B: pregnant women with late-onset preeclampsia(LOPE), age(20-35), gestational weeks(34 weeks-37 weeks, including 34weeks). Group C: pregnant women with late-onset preeclampsia(LOPE), age(20-35), gestational weeks(more than 37 weeks, including 37weeks). Group D: normal pregnant women, age(20-29), gestational weeks(20 weeks-34 weeks, including 20 weeks). Group E: normal pregnant women, age(21-30), gestational weeks(34 weeks-37 weeks, including 34weeks). Group F: normal pregnant women, age(20-36), gestational weeks(37 weeks-42 weeks, including 37 weeks and 42 weeks).We need 3ml venous and 3ml urinary within 24 hours. ELISA assay is used to detect the expression of blood and urine.3 Use immunohistochemical method to detect the expression of NGAL in placenta tissueThe Specimen are divided into 3 groups. Group 1: pregnant women with early-onset preeclampsia(EOPE), age(20-35), gestational weeks(20 weeks-34 weeks, including 20 weeks). Group 2: pregnant women with late-onset preeclampsia(LOPE), age(20-35), gestational weeks(34 weeks-37 weeks, including 34weeks). Group 3: normal pregnant women, age(20-36), gestational weeks(37 weeks-42 weeks, including 37 weeks and 42 weeks).The placentas we need is about 2.0 cm×l.0 cm×1.0 cm(avoid hemorrhage, infarct and calcification) which is taken from the maternal surface of placental within 2 minutes post partum. Washed by normal saline for three times, it is then put in the stationary liquid in pathology bag and marked for later use. All the process requires sterility and the specimen are put into-80°C refrigerator.4 We use SPSS 13.0 software to analyse the results and P<0.05 is statistically significant difference.Results:1 The content and comparison of serum NGAL between pregnant women with early-onset preeclampsia, pregnant women with late-onset preeclampsia and normal pregnant womenThe serum NGAL in group A is 1.635±0.1004g/L, and the serum NGAL in group C is 0.723±0.1124g/L; the serum NGAL is significant different in the two groups(P<0.01). The serum NGAL in group B is 1.290±0.0845g/L, and the serum NGAL in group E is 0.725±0.0539g/L, the serum NGAL is significant different in the two groups(P<0.01). The serum NGAL in group C is 1.269±0.1173g/L, and the serum NGAL in group F is 0.705±0.1094g/L; the serum NGAL is significant different in the two groups(P<0.01). There are significantly difference in serum NGAL in group A, group B and group C(P<0.05) except the comparison of group B and group C(P>0.05).2 The content and comparison of urine NGAL between pregnant women with early-onset preeclampsia, pregnant women with late-onset preeclampsia and normal pregnant women The urine NGAL in group A is 0.470±0.069g/L, and the urine NGAL in group D is 0.141±0.024g/L; the urine NGAL is significant different in the two groups(P<0.01). The urine NGAL in group B is 0.258±0.042g/L, and the urine NGAL in group E is 0.102±0.006g/L, the urine NGAL is significant different in the two groups(P<0.01). The urine NGAL in group C is 0.249±0.041g/L, and the urine NGAL in group F is 0.116±0.020g/L; the urine NGAL is significant different in the two groups(P<0.01). There are significantly difference in urine NGAL in group A, group B and group C(P<0.05) except the comparison of group B and group C(P>0.05).3 The content of placenta tissue NGAL between pregnant women with early-onset preeclampsia, pregnant women with late-onset preeclampsia and normal pregnant womenVisible NGAL protein positive expression was observed in the cytoplasm of Form-fitting sertoli cell In placenta villi, tan in color. The NGAL’s expression in placenta tissue of normal group, EOPE group and LOPE group is respectively 10.15±0.86g/L, 50.90±7.60g/L, and 30.51±4.16g/L. Compared with normal group, the NGAL’s expression in placenta tissue is higher in EOPE group and LOPE group(P<0.01). There are significantly difference in placenta tissue NGAL in EOPE group and in LOPE group(P<0.05).Conclusion:1 The content of NGAL in serum and urine has been increased in EOPE patients and LOPE patients compared with normal control group at the same time, and EOPE patients NGAL protein content increased significantly.2 The content of NGAL in placenta tissue has been increased in EOPE patients and LOPE patients compared with normal control group, and EOPE patients NGAL protein content increased significantly.