| Malignant tumor occurrence and development is related to proliferation. Malignant cells are parasitic in the body, the body is the internal environment which they depend for survival. Therefore, the study of changes in peripheral blood mononuclear cells may provide a theoretical basis for further discussion on the relationship between malignant tumor cells and body. Peripheral blood mononuclear cells, including lymphocytes and monocytes, lymphocytes including T lymphocytes, B lymphocytes and natural killer cells, plays an important role in immune surveillance, killing target cells and immune regulation, has an extremely important role in immune surveillance, kill target cells and immune regulation aspects, is the body’s important effector cells to anti-tumor immunity of the body,the number and functional state of peripheral blood mononuclear cells reflect the body’s systemic immune function level. IL-8 is a multicellular origin, multifunctional cytokine, can promote the inflammatory response, stimulate angiogenesis, promote mitosis, regulating host immune function effect,is highly expressed in a variety of tumors, and is closely related to the.tumor progress and early event occurrence. Flow cytometry is a quantitative analysis and sorting of single cells or other biological particles in function level detection means, is the most advanced and the most accurate technique on the detection of cell differentiation and function in the current,is mainly used in the precancerous lesions and early diagnosis of tumor, cross matching, the determination of bone marrow hematopoietic stem cells reconstruction, lymphocyte subsets analysis,detection and analysis of cell differentiation and apoptosis and DNA cycle and so on. Chemotherapy is one of the main treatment for malignant tumors, in addition has the function of killing tumor cell, but also damage normal tissue cells.Objective: To investigate the intervention in patients with malignant tumor chemotherapy on peripheral blood mononuclear cell function influence.Method: Flow cytometry was used to detect the cell cycles and apoptosis rate of peripheral blood mononuclear cells(PBMC) and IL-8 levels in 43 patients with malignant tumors and 10 normal healthy subjects morning fasting peripheral venous blood.Results:1 Comparison of peripheral blood mononuclear cell function between normal healthy subjects and patients with malignant tumors before chemotherapy.Patients with malignant tumor before chemotherapy compared with normal healthy subjects: the percentage of G0/G1 phase of PBMC was decreased,S phase increased, G2/M phase increased,apoptosis rate increased, IL-8 increased, the difference was not statistically significant(P> 0.05).2 Comparison between normal healthy subjects and patients with malignant tumors after chemotherapy. Patients with malignant tumor after chemotherapy compared with normal subjects: 1) the percentage of G0/G1 phase was decreased(86.06 ± 9.08) :( 93.18 ± 4.07), the percentage of G2 / M phase increased(11.51 ± 8.27) :( 4.00 ± 2.24), the difference was statistically significant(P<0.05). 2) S phase increased, IL-8 increased, apoptosis rate increased,but the difference was not statistically significant(P> 0.05).3 Comparison between normal healthy subjects and small load group of patients with malignant tumors after chemotherapy. 1) After chemotherapy,in small load group the percentage of G0/G1 phase was decreased(85.64 ± 9.85):( 93.18 ± 4.07), the percentage of G2 / M phase increased(12.11 ± 8.45):( 4.00 ± 2.24), the apoptosis rate increased(16.90 ± 18.24) :( 2.90 ± 1.87), the difference was statistically significant(P<0.05). 2) S phase Increased, IL-8 increased, but the difference was not statistically significant(P> 0.05).4 Comparison between normal healthy subjects and large load group of patients with malignant tumors after chemotherapy. 1) After chemotherapy, in large load group the percentage of G0/G1 phase was decreased(86.62 ± 8.33) :( 93.18 ± 4.07), the percentage of G2 / M phase increased(10.71 ± 8.33) :( 4.00 ± 2.24), the difference was statistically significant(P <0.05). 2) S phase decreased, IL-8 increased, apoptosis rate increased but the difference was not statistically significant(P> 0.05).5 Comparison between patients with malignant tumors before chemotherapy and after chemotherapy.1) After chemotherapy, the percentage of G0/G1 phase was decreased, the percentage of G2 / M phase increased, IL-8 decreased. The difference was not statistically significant(P> 0.05). 2) After chemotherapy, apoptosis rate of PBMC was increased(12.63 ± 18.09) :( 4.68 ± 8.88), was statistically significant(P <0.05).6 Comparison between small load group of patients with malignant tumors before chemotherapy and after chemotherapy.1) After chemotherapy decreased, the percentage of G0/G1 phase was decreased,S phase decreased, the percentage of G2 / M phase increased, IL-8 increased, but the difference was not statistically significant(P> 0.05). 2) After chemotherapy,apoptosis rate of PBMC increased(16.90 ± 18.24) :( 4.45 ± 7.21), the difference was statistically significant(P <0.05).7 Comparison between large load group of patients with malignant tumors before chemotherapy and after chemotherapy.After chemotherapy, the percentage of G0 / G1 phase decreased, S phase increased, G2 / M phase increased, apoptosis rate of PBMC increased, IL-8 decreased,the difference was not statistically significant(P> 0.05).8 Comparison between subgroups of patients with malignant tumor in PBMC function according to the tumor load before chemotherapy. The large load subgroup was increased than the small load subgroup in the percentage of IL-8(4.54 + 3.83):(2.06 + 2.14), the difference was significant(P<0.05). The proportion of G0/G1 phase increased, S phase rate decreased, increased G2/M ratio, increased apoptosis rate, the difference was not statistically significant(P> 0.05).9 Comparison between subgroups of patients with malignant tumor in PBMC function according to the tumor load after chemotherapy. The large load subgroup was increased than the small load subgroup in the percentage of IL-8 The proportion of G0/G1 phase increased, S phase rate decreased, G2/M ratio decreased, apoptosis rate decreased, the difference was not statistically significant(P> 0.05).Conclusion:1 Normal healthy subjects and patients with malignant tumor before chemotherapy on peripheral blood mononuclear cell cycle, apoptosis rate and IL-8 level had no significant difference.2 Patients with malignant tumor after chemotherapy compared with normal subjects on peripheral blood mononuclear cell cycle,the percentage of G0/G1 phase was decreased,the percentage of G2 / M phase increased.3 The apoptosis rate of peripheral blood mononuclear cells can be increased after chemotherapy intervention, the blood time of scientific research before chemotherapy is recommended.4 The IL-8 was significantly higher in the large load subgroup of patients with malignant tumor before chemotherapy, which indicated that IL-8 could reflect the function of peripheral blood mononuclear cells.5 Chemotherapy can significantly change the cells cycle and apoptosis of peripheral blood mononuclear cells in patients with malignant tumor, so as to provide a theoretical basis for blood sample time of the follow-up study and immune function detection. |
