GIK’s Therapeutic Intervention For Mice And Effect On Spleen PI3K Protein Expression

Objectives: We tried to explore the damage of endotoxin to spleen functions by establishing a mice model of endotoxemia, and then, explored how GIK alleviated endotoxin-induced spleen damage and its possible mechanism by giving the model mice Glucose, insulin- potassium solution(GIK) treatment, as well as detecting spleen PI3 K protein expression in endotoxemia model mice.Methods: Sixty BALB / c male mice(SPF level) aging 8-10 weeks were randomly divided into four groups using random number table. The control group(n = 10): no treatment, free access to food; NS group(n = 10):, three days of intraperitoneal injection of saline 5ml / kg / d continuously, free access to food; LPS group(n = 20): three days of intraperitoneal injection of LPS 5ml / kg / d continuously, free access to food; GIK group(n = 20): three days of intraperitoneal injection of LPS 5ml / kg / d firstly, and tail intravenous injection of GIK 20 ml / kg / d followed by, continuously injection of three days, free access to food. We respectively weighed the initial body weight(T0)of mice in the control group, NS group, LPS group and GIK group at the beginning of the experiment, and then the mice in each group were given the appropriate treatment in accordance with the experimental design, and were weighed at 24h(T1), 48h(T2), and 72h(T3). After weighing at 72h(T3), the mice were sacrificed using cervical spinal break method, and the wet spleen weights were recorded. Mice weight and spleen weight of each group were recorded in the four consecutive days, then spleen indexes were calculated, and then spleen PI3 K protein expressions were tested using Western Blot method, and finally the results were statistically analyzed.Results: 1, Mice in the control group and NS group all had steady breathing, dry hair, quick response, and normal defecate and urinate, no special events, and no deaths. After intraperitoneal injection of LPS, mice in LPS group appeared reducing activity, drowsiness, lethargy, piloerection, jitter, and shortness of breath, rapid heart rate, cyanosis, loose stools, etc. Mice in GIK group had quickening breathing, weakened activity, and loose stools, etc. And their overall performances were better compared with LPS group.2, Weight comparison: I, initial body weight(T0): Paired-comparisons of initial body weight(T0) in control group, NS group, LPS group and GIK group had no significant difference; Ⅱ, 24h(T1) Weight: ①There was no significant difference in body weight compared NS group with the control group, and the mice weight in LPS group and GIK group was significantly lighter(P <0.05)compared with the control group; ②the mice weight in LPS and GIK group was significantly lighter compared with the NS group(P <0.05). ③the mice weight in LPS group was significantly lighter compared with GIK group(P <0.05);III, 48h(T2) Weight: ①There was no significant difference in body weight compared NS group with the control group, and the mice weight in LPS group and GIK group was significantly lighter(P <0.05)compared with the control group; ②the mice weight in LPS and GIK group was significantly lighter compared with the NS group(P <0.05). ③the mice weight in LPS group was significantly lighter compared with GIK group(P <0.05);IV, 48h(T3) Weight: ①There was no significant difference in body weight compared NS group with the control group, and the mice weight in LPS group and GIK group was significantly lighter(P <0.05)compared with the control group; ② the mice weight in LPS and GIK group was significantly lighter compared with the NS group(P <0.05). ③the mice weight in LPS group was significantly lighter compared with GIK group(P <0.05);3, Spleen weight comparison: ①There was no significant difference between the control group and the NS group, and spleen weight in LPS group and GIK group was significantly lighter than in the control group(P <0.05). ②spleen weight in LPS group and GIK group was significantly lighter than in the NS group(P <0.05). ③spleen weight in LPS group was significantly lighter than in GIK group(P <0.05).4, Spleen index comparison: ①spleen index in control group and the NS group showed no significant difference, and spleen index in LPS group and GIK group was significantly lower than in the control group(P <0.05). ②spleen index in LPS group and GIK group was significantly lower than the NS group(P <0.05). ③spleen index in LPS group was significantly lower than in GIK group(P <0.05);5, PI3 K protein: ①PI3K expression in control group and NS group showed no significant difference, PI3 K protein expression in LPS group and GIK group increased than in the control group(P <0.05). ②PI3K protein expression in LPS group and GIK group increased(P <0.05) than in the NS group. ③PI3K protein expression in LPS group increased significantly than in GIK group(P <0.05).Conclusions: Intraperitoneal injection of endotoxin could induce weight loss, decreased spleen weight and increased spleen index in mice, and spleen damage resulted in spleen dysfunction and the expression of PI3K enhancement. After GIK treatment, significantly improvement were observed in GIK treated mice compared with the untreated mice in daily activities, spleen weight, PI3 K protein expression, etc, which indicated that GIK had some therapeutic effect on LPS-induced spleen damage, and this effect might be associated with the inhibition of PI3 K protein expression.