MMP-13 In The Lumbar Disc Herniation Expression Of Nucleus And Serum

Objective: Disc disease is common orthopedic disease, seriously affecting people’s daily lives, their lumbar disc degeneration is considered the most common cause. A series of domestic and international studies have confirmed the composition and amount of changes in the extracellular matrix are the main causes leading to the degeneration of the intervertebral disc mechanical characteristics changed. Family of matrix metalloproteinases(MMPs) play an important role in the degradation of extracellular matrix in the disc. Studies have shown that a variety of matrix metalloproteinases can degrade type II collagen, but the maximum matrix metalloproteinase-13(MMP-13) in the degradation efficiency, which play an important role. Lumbar disc herniation of intervertebral disc degeneration as common, and clinical article by peripheral blood serum levels of MMP-13 expression, to explore its possible role in the process of disc degeneration and compare trauma and lumbar disc herniation and scientific significance. Discussion of lumbar disc herniation type, extrusion type and normal lumbar disc and the difference in serum levels of expression of MMP-13.Methods: The 2014 April-December in the Affiliated Hospital of North Sichuan Medical College Orthopaedics for "lumbar disc herniation" admission, the patient with a medical team in elective discectomy or internal fixation of randomly selected 31 cases included in the experimental group, and further by CT, MRI imaging data and surgical findings are further divided into herniated nucleus and nucleus-type extrusion-type groups, and the other will the same period 12 cases previously healthy, due to trauma caused by lumbar fractures(burst) parallel anterior cut open reduction and internal fixation of patients satisfied the control group. Immunohistochemistry was used to detect the three groups of patients nucleus pulposus matrix metalloproteinase-13(MMP-13) expression, preclude the use of Nikon BM-1 multifunctional biological microscope at 100-200 high-power digital camera field of view are taken Fromowitz positive cells were observed by semi-quantitative number of positive cells, and calculate the positive rate. ELISA was used to detect blood serum of three groups of patients MMP-13 expression.Results: 1.Expression control disc tissue MMP-13 was weakly positive or obscure, the average positive rate was(17.3 ± 2.3)%; expression degenerative lumbar disc herniation group tissue MMP-13 was focal or diffuse strong staining, which highlights the positive expression group, the average rate((73.3 ± 10.6))%, prolapse group(71.5 ± 9.3)%. MMP-13 in the experimental group was significantly higher positive rate(P <0.05). Tip MMP-13 may be in lumbar disc degeneration lumbar disc herniation in particular play an important role in the pathogenesis. 2.MMP-13 was no significant difference in the prominent degeneration, prolapse of lumbar intervertebral disc, suggesting high levels of MMP-13 may continue to exist in the disc degeneration up to a certain extent, it may in the pathogenesis of lumbar disc degeneration plays an important role.3.Trauma and lumbar disc herniation serum MMP-13 expression had a certain degree, lower expression(control group) and MMP-13 in the control group disc tissue concentrations of MMP-13 average was 9.745 ± 2.82 ng / ml; prominent group of degenerative disc tissue concentrations of MMP-13 average was 15.436 ± 5.156 ng / ml. MMP-13 average concentration of degenerative prolapse group was 16.241 ± 4.072 ng / ml in the experimental group P<0.05, the difference was statistically significant; prominent group and prolapse group compared with the control group(P>0.05), the difference was not statistically significant, suggesting that MMP-13 expression is closely related with disc degeneration in the serum. 4.The control group in serum MMP-13 average concentration was 9.965 ± 2.42 ng / ml, in the control group(female) in serum MMP-13 average concentration was 9.23 ± 2.398 ng / ml the comparison(P> 0.05), the difference was not statistically significant; the experimental group(M) in serum concentrations of MMP-13 average was 15.528 ± 4.469 ng / ml experimental group, the average concentration(female) in serum MMP-13 was 16.119 ± 5.093 ng / ml, which Compare(P> 0.05), the difference was not statistically significant, than no significant relationship prompted human serum MMP-13 expression and sex.Conclusions: 1.Trauma and lumbar disc herniation prominent nucleus pulposus prolapse type MMP-13 expression had a certain degree, but in prominent prolapse group was significantly increased.Tip abnormal expression of MMP-13 may in particular disc degeneration lumbar disc herniation, which play an important role. 2.MMP-13 in the lumbar disc herniation nucleus pulposus, no significant difference in the nucleus pulposus prolapse, suggesting high levels of MMP-13 may continue to exist in the disc degeneration up to a certain extent, it might lumbar disc degeneration in the pathogenesis play an important role. 3.Trauma and lumbar disc herniation, MMP-13 expression had a certain degree of prolapse and trauma type serum, but it was significantly higher expression in the lumbar disc herniation group, MMP-13 in prominent prolapse serum no significant difference. Trauma patients in combination with increasing age elevated expression of MMP-13, suggesting that serum MMP-13 expression was positively correlated with the base, and disc degeneration to some extent continuing high level of presence, which may in the incidence of lumbar disc degeneration which play an important role. 4.The expression of MMP-13 in serum and no significant relationship between sex ratio.