Effect Of Medicated Food On Intestinal Mechanical Barrier For Rats With Chemotherapy-induced Gastrointestinal Mucositis Based On The ERK/MAPK Pathway

ObjectiveTo explore the effect of Medicated Food on Intestinal Mechanical Barrier for Rats with Chemotherapy-induced Gastrointestinal Mucositis based on the MAPK/ERK pathway.MethodsForty-eight SPF Wistar rats were completed randomly assigned to intervention group, model group, and control group with 16 rats in each group. A total of 15 days, on the 7th day of study, rats in intervention and model groups were injected with 5-FU at 150 mg·kg-1 to reproduce the model of chemotherapy-induced gastrointestinal Mucositis. Rats were killed on the 1st and 8th day after chemotherapy to investigate the level of expressions of intestinal tight junction proteins(Occludin, Claudin-1) and channel proteins(ERK1/2、p-ERK1/2、Ras、MEK) by Western blot. Statistical software SPSS 18.0 was applied for data analysis.Results1 The change of intestinal tight junction proteins in ratsExpressions of Occludin and Claudin-1 in model group were all lower than those in intervention and control groups on the 1st day after chemotherapy. Claudin-1 expressions in model group were significantly lower than control group(P<0.01). Occludin expressions in model group were significantly lower compared with intervention and control groups(P<0.01).On the 8th day after chemotherapy, Claudin-1 expressions in model group were significantly lower compared with intervention and control groups(P<0.05 or 0.01). Occludin expressions in model group were significantly lower than control group(P< 3.05).2 The change of channel proteins in ratsOn the 1st day after chemotherapy, expressions of channel proteins in control group were all lower than other groups. Ras expressions in model grouo showed significant change compared with intervention group(P<0.01).On the 8th day after chemotherapy, expressions of channel proteins showed significant change among groups(P<0.05 or 0.01). ERK1/2 expressions in model group showed significant change among intervention and control groups(P<0.01).3 The change of Spleen deficiency symptom scores in ratsOn the 1st to 7th day of study, each group of Spleen deficiency symptom scores showed no significant change. Scores began to increase from the 1st day after chemotherapy in intervention and model groups, and the 3rd day were most obvious. Although both intervention and model groups showed a negative change in scores, the scores in intervention group was still better than that in model group.4 The change of body weight and food intake in rats4.1 Body weight On the 1st to 7th day of study, each group of body weight showed no significant change. Body weight in intervention and model groups were decrease from the 1st to 3rd day after chemotherapy, and then, improvement was detected in these two groups. The decreasing trend of body weights in intervention group was still lower than that in model group.4.2 food intake Food intake were significantly decreased in intervention and model groups from the 1st to 3rd day after chemotherapy compared with control group. Although both intervention and model groups showed a decreasing trend in food intake, the change in intervention group was still lower than that in model group.ConclusionQi Yao Ji Jin Zhou medicated food has a positive impact on protecting the mechanical barrier of intestinal tract and maintaining the expression of tight junction proteins, through the ERK/MAPK pathway probably.