| [Objective]To investigate the role of "Qi Yao Ji Jin Zhou" medicated food in the protection of rat models with5-fluorouracil (5-FU) induced intestinal biological barrier.[Methods]Forty-eight male Wistar rats were randomly assigned to the model group, the intervention group, and the control group. During the14days, the intervention group daily fed with medicated food at10ml/kg,while the model and the control groups administrated with an equal amount of physiological saline solution. On the7th day, rats in the model and the intervention groups were injected with a single dose of5-FU at150mg/kg to produce the model of chemotherapy-induced gastrointestinal mucositis.With the intervention periods, the general conditions, body weight of rats were recorded. Rats were killed on the1st and8th day after chemotherapy,in order to investigate the flowing indicators:1) the level of endotoxin in blood;2) the level of plasma D-lactic acid;3) the effect of bacterial translocation;4) the change of body weight and the general conditions.Statistical software SPSS17.0was applied for data analysis.1The level of endotoxin in bloodOn the1st day after chemotherapy,rats in the model group had higher endotoxin than control group (P<0.01),while lower endotoxin than the intervention group (P<0.05).On the8th day after chemotherapy,the level of endotoxin in the intervention group (P<O.05) and the control group (P<0.01) were significantly lower when compared with the model group.2The level of plasma D-lactic acid.On the1st day after chemotherapy, compared with the model group, the level of plasma D-lactic acid was significantly lower in the control group (P<0.01) and the intervention group (P<0.05).On the8th day after chemotherapy, the level of D-lactic acid reached statistical significance after the comparison between the model and intervention group, the model and the control group, the model group had higher level D-lactic acid than the intervention group (P<0.01),while the control group was significantly lower than the model (P<0.05)3The change of intestinal bacterial translocation On the1st day after chemotherapy, the bacterial translocation to liver, spleen and mesenteric lymph nodes in model group were all higer than those in the control group (P<0.05).After comparison between the model and the intervention group, only the bacterial translocation to liver reached statistical significance (P<0.05). Although the bacterial translocation to spleen and mesenteric lymph nodes in the model group were higher than the intervention group, it had no significant difference (P=0.132>0.05, P=0.119>0.05)On the8th day after chemotherapy, while compared with the model group, the bacterial translocation of spleen and mesenteric lymph nodes were all respectively higer than those in the control group (P<0.05), although the bacterial translocation of liver was higer than those in the control group,there was no statistical significance (P=0.077>0.05). After comparison between the intervention and the model group, significantly difference was only showed in the bacterial translocation to mesenteric lymph nodes (P<O.05),no statistical significance were fund on liver and spleen (P=0.077>0.05, P=0.132>0.05)4The change of body weight and general conditionDuring the expremintal day, body weight in the control group increased daily while others also showed a normal grown before the chemotherapy. Weight on the model group and intervention group were increased slowly at the beginning of chemotherapy, weight-loss was occurred from the1st day after chemotherapy in the two groups,and it dropped to the lowest level on the3th day after chemotherapy,then slightly increased in late time.General condition was better among the control and intervention groups than the model group, the model group showed a negative change in the overall of daily general conditions,such as squinting, sleepiness,poor appetite, weakness on activities, dark hair and diarrhea.These symptoms of spleen deficiency prompted that5-FU induced the intestinal biological barrier in rats.[Conclusion]Rats treated with the injection of5-FU intraperitoneal can successfully find the model of gastrointestinal mucositis.The Qi Yao Ji Jin Zhou medicated food had obviously positive impact on protecting the dysfuction of intestinal biological barrier, resulted in relieving the deterioration of general conditions and weight-lose, and reducing the level of D-lactic acid and endotoxin. It also had the potential to prevent and relieve the bacterial translocation after5-FU induced chemotherapy. |
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