Expression And Clinical Significance Of Bmi-1 And Mina53 In Colorectal Carcinoma

ObjectiveBelong to tumors of digestive system, colorectal carcinoma is one of the common malignant tumors. The morbidity of colorectal carcinoma are quite different in different areas. The morbidity of colorectal carcinoma is much higher in Nourh America and Western Europe and other developed countries, than that of Africa and Asia. The morbidity of colorectal carcinoma is also relatively low in our country. The morbidity and mortality of colorectal carcinoma in our country has rose in recent years as diet habits westernized, lifestyle changed and living standards improved. Therefore, colorectal carcinoma has become one of the main diseases threatening people’s health and life. So people should pay more attention to research new ways to prevent and treat colorectal carcinoma. But the cause and pathogenesis of colorectal carcinoma is not entirely clear at the moment. Most domestic and international experts believe that this disease is a complicated course under the effect of environmental factors and genetic factors involving multi-stop, multistage and multigene. B-cell-specific moloney murine leukemia virus insertion site 1 is a number of polycomb group genes. Bmi-1 participates in the formation and development of tumor via multiple signal pathways. It is confirmed that Bmi-1 is related to the emergence and development of multiple tumors such asnasopharyngeal carcinoma, breast cancer, lung cancer and prostate cancer. Myc-induced nuclear antigen with a molecular mass of 53 k Da is one of the downstream target genes of c-myc, which is a newly discovered proto-oncogene in recent years. Mina53 can promote tumor proliferation and differentiation via multiple mechanism. Bmi-1 and Mina53 express anomaly in a wide variety of tumors, but their pathogenesis in colorectal carcinoma is not very clear, and not only the relevant reports of the study of their expression in colorectal cancer but also the study of their correlation are rarely seen at home and abroad now. This study is designed to detect the expression of Bmi-1 and Mina53 protein in 56 cases of colorectal carcinoma and normal colorectal mucosa by immunohistochemical method, and investigate their relationship in the development of colorectal carcinoma and their clinical pathological significance.Methods1. Streptavidin perosidase is used to detect the expression of Bmi-1 and Mina53 proteins in fifty six cases of colorectal carcinoma and fifty six cases of normal colorectal mucosa tissues, and analyze the relationship between the proteins expression and clinical factors such as sex, age, lymphatic metastasis, differentiation, and analyze the relevance of their expressions.2. SPSS17.0 statistical package is used to analyze all the datas, and statistical methods not only consist of the Chi-square test, but also contain Spearman correlation ananlysis, and at the moment the difference is statistically significant when P<0.05.Results1. Immunohistochemistry results showed that: the positive rate of Bmi-1 in fifty six cases of colorectal carcinoma tissues was 80.4%(45/56),but it was 35.7%(20/56)in fifty six cases of normal colorectal mucosa tissues, statistically significant difference(χ2=22.913,P<0.05).2. The expression of Bmi-1 had no statistically significant difference in age or sex(P>0.05),however, there was significant association in tumor invasion, lymphatic metastasis and differentiation(P<0.05).3. Immunohistochemistry results showed that: the positive rate of Mina53 in fifty six cases of colorectal carcinoma tissues was 73.2%(41/56),but it was 19.6%(11/56)in fifty six cases of normal colorectal mucosa tissues, statistically significant difference(χ2=32.308,P<0.05).4. The expression of Mina53 had no statistically significant difference in age or sex(P>0.05),however, there was significant association in tumor invasion, lymphatic metastasis and differentiation(P<0.05).5. Statistical analysis showed that a strong postive relationship between the expression of Bni-1 and Mina53 protein in colorectal carcinoma(r=0.269, P<0.05).Conclusions1. The expression of Bmi-1 in colorectal carcinoma is high, and it is related to tumor invasion, lymphatic metastasis and differentiation, which indicate that high expression of Bmi-1 may have a hand in the development and metastasis in colorectal carcinoma. 2. The expression of Mina53 in colorectal carcinoma is high, and it is related to tumor invasion, lymphatic metastasis and differentiation, which indicate that high expression of Mina53 may have a hand in the development and metastasis in colorectal carcinoma. 3. The correlation of the expression of Bmi-1 and Mina53 is positive, which indicate that Bmi-1 may regulate the expression of Mina53 through a mechanism, and that high expression of them may have a hand in the development and metastasis of colorectal carcinoma together, and that combined detection of Bim-1 and Mina53 may has important implications for the diagnosis and prognosis of colorectal carcinoma.