Expression And Significance Of Mina53and Mel-18in Colorectal Carcinoma

ObjectiveColorectal cancer is one of the most common digestive system malignant tumor, morbidity of which ranks fifth in malignant tumor in our country. In recent years, with increasing of life level and changing of diet habit, morbidity and mortality of colorectal carcinoma in our country is gradually increasing, which is a serious threat to people’s health. Therefore, to explore the pathogenesis of colorectal carcinoma and to seek effective prevention and treatment approaches, is a problem to be solved. Proto-oncogene Myc family widely participate in the regulation of cell proliferation, by which transcription and expression of multiple genes are activated. Mina53, as a nuclear antigen gene,is directly induced by c-myc,a member of the family.It highly expresses in a variety of tumor tissue. Polycomb group plays a key role on embryo formation, hematopoietic and regulating of cell cycle in mammalian. Mel-18,as a member of polycomb group, in a variety of tumor tissues expresses lowly or don’t express. At present at home and abroad the researches of Mina53and Mel-18little has been reported,and there is no research about expresions of the two in colorectal carcinoma and their relationship. By detecting expression of Mina53and Mel-18protein in colorectal carcinoma tissue, this experiment studies their relationship with clinical pathological features of patients with colorectal cancer and discusses expression and significance of Mina53and Mel-18in colorectal carcinoma.MethodsThe expression of Mina53and Mel-18were detected by immunohistochemistry in60colorectal carcinoma specimens and60normal colorectal mucosa specimens. The patients’clinicopathological parameters were collceted and anlysised the relationship between Mina53and Mel-18protein in colorectal cancer pathological factor. The data were analyzed by SPSS13.0for windows.Results1.Mina53and Mel-18positive dyed yellow grain, both located in the nucleus. It is obvious heterogeneity. Mina53strongly expresses in tumor tissues but don’t express in normal colon mucosa tissue adjacent to carcinoma. The expression, rate of Mina53protein in colorectal carcinoma and normal tissue is60.0%and0respectively, differences between groups statistically significant (P<0.05). The expression of Mel-18in tumor tissues is relatively weak (or negative), and in normal colon mucosa tissues adjacent to carcinoma has strong expression. The expression rate of Mel-18protein in colorectal carcinoma and normal tissue is31.7%and61.7%respectively, differences between groups statistically significant (P<0.05).2.Expression of Mina53in colocectal carcinoma tissues has nothing to do with the patient’s gender and age (P>0.05), and is related with infiltration depth, lymph node metastasis and differentiation degree of tumor(P<0.05).3. A negative correlation was noted between the expression of Mina53and that of Mel-18in colorectal carcinoma.Conclusions1. Mina53protein in colorectal carcinoma tissue expresses highly and has nothing to do with patient’s sex and age and is related with infiltration depth, lymph node metastasis and the differentiation degree of tumor. High expression of Mina53protein may be involved in the development of colorectal carcinoma and it’s metastasis process.2. Mel-18in colorectal carcinoma tissue expresses lowly or don’t express and has nothing to do with patient’s sex and age and is related with infiltration depth, lymph node metastasis and the differentiation degree of tumor.Low expression of Mel-18may be involved in the development of colorectal carcinoma and its metastasis process.3. A negative correlation was noted between the expression of Mina53and that of Mel-18in colorectal carcinoma.Expression of Mina53together with Mel-18may play an important role in development of colorectal carcinoma.