Research On Expression Of C-Myc And CD24in Colorectal Carcinoma And Colorectal Polyps

Objective To analyse the role of c-myc and CD-24gene in the carcinogenesis of colorectal carcinoma and the correlation between them,we determined the expression of c-myc and CD-24in colorectal carcinoma,colorectal Polyps and normal mucosa.Methods The expression of c-myc and CD24in colorectal carcinoma (n=60), colorectal Polyps (n=60), and the adjacent non-cancerous tissues(n=30) was observed by immunohistochemical assay.Results (1)Positive expression of c-myc were found in73.3%colorectal carcinoma, which was significantly higher than that in colorectal adenomatous polyps(44.4%), colorectal hyperplastic polyps(13.3%) and adjacent non-cancerous tissues(5%). The expression of c-myc in colorectal adenomatous polyps was higher than that in colorectal hyperplastic polyps and adjacent non-cancerous tissues.The expression of c-myc had a significant correlation with the tumor location, lymph node metastasis, Dukes’staging and tumor differentiation.There was a highly expression rate of c-myc in distal colon/rectum, Lymph node metastasis, Dukes stage(C+D) and Poorly differentiated carcimoma (P<0.05或P<0.01). There was no significant correlation between the c-myc immunoexpression and patient’s age,gender, tumor diameter, tumor Growth pattern, tumor Distant metastasis and invasive depth (P>0.05).There was a highly expression rate of c-myc in colorectal adenomatous polyps(polyp diameter>lcm or without Pedicle)(P<0.05或P<0.01),but it didn’t correlate with patient’s age,gender and adenomatous polyps’location (P>0.05)(2) Positive expression of CD24were found in76.7%colorectal carcinoma, which was significantly higher than that in colorectal hyperplastic polyps(6.7%) and adjacent non-cancerous tissues(3.3%),but it had no significant difference with that in colorectal adenomatous polyps(66.7%). The expression of CD24in colorectal adenomatous polyps was higher than that in colorectal hyperplastic polyps and adjacent non-cancerous tissues. The expression of CD24had a significant correlation with the tumor diameter, lymph node metastasis, Dukes’ staging and tumor differentiation.There was a highly expression rate of CD24in>5cm, Lymph node metastasis, Dukes stage(C+D) and Poorly differentiated carcimoma (P<0.05或P<0.01). There was no significant correlation between the CD24immunoexpression and patient’s age,gender, tumor location, tumor Growth pattern, tumor Distant metastasis and invasive depth (P>0.05). There was a highly expression rate of CD24in colorectal adenomatous polyps(polyp diameter>lcm or without Pedicle)(P<0.05),but it didn’t correlate with patient’s age,gender and adenomatous polyps’location (P>0.05)(3)The positive expression rate of CD24was significant higher than that of c-myc in colorectal adenomatous polyps (P<0.05).There was a positive correlation relationship between CD24and c-myc in colorectal carcinoma tissue.Conclusion (1)Both c-myc and CD24participated in the carcinogenesis and development of colorectal carcinoma. The expression of c-myc had a significant correlation with the tumor location, lymph node metastasis, Dukes’staging and tumor differentiation, The expression of CD24had a significant correlation with the tumor diameter, lymph node metastasis, Dukes’staging and tumor differentiation.(2) There was a highly expression rate of c-myc and CD24in colorectal adenomatous polyps,which meant that both of them may play a role in the canceration or even happenness of the colorectal adenomatous polyps, There was significant correlation between them and the size or Pedicle of colorectal adenomatous polyps.They may be one of the early event in the carcinogenesis of colorectal carcinoma.(3)CD24may be an earlier event in the carcinogenesis of colorectal carcinoma than c-myc, There was a positive correlation relationship between CD24and c-myc in colorectal carcinoma tissue,they probably played a cooperative role in the carcinogenesis, progression and metastasis of colorectal carcinoma.