Changes Of SP-D And Gal-9 In Serum And Cerebrospinal Fluid Of Children With Viral Encephalitis

Objective:Investigating the function of SP-D and Gal-9 in the pathogenesis of; viral encephalitis as well as the potential significance of both in the diagnosis and prognosis of VE.Methods:Children admitted in Pediatric Neurology from July to September 2014 and diagnosed with VE as objects in our research were chosen as experimental group. We chosen children with obstructive hydrocephalus and children with acute lymphoblastic leukemia in remission were categorized into control group. Then we collected serum and cerebrospinal fluid specimens from both children with VE and control group in convalescent period and acute period respectively, then the specimens were preserved at minus 80 degrees Celsius after pretreatment. We detected the levels of SP-D and Gal-9 in the serum and cerebrospinal fluid by double antibody sandwich enzyme-linked immunosorbent assay. SPSS21.0 statistical software was used to do statistical analysis later.Results:1. There was no significant differences in sex and age between VE group and control group.2. The differences of SP-D levels in serum and cerebrospinal fluid were statistically significant between acute phase group and recovery phase group of VE as well as control group (F= 103.58、118.15, P<0.01). The levels of SP-D in serum and cerebrospinal fluid of the children in VE acute phase and of the children in VE recovery phase were significantly lower compared to the control group(P<0.01). The levels of SP-D in serum and cerebrospinal fluid of children in VE convalescent phase were significantly higher than those in acute phase of VE (P<0.01). There was a positive correlation between levels of SP-D in cerebrospinal fluid and in serum(r=0.589, P<0.01).3. The differences of Gal-9 levels in serum and cerebrospinal fluid were statistically significant between VE acute phase group and VE recovery phase group as well as control group (F=92.70、81.95, P<0.01). The levels of Gal-9 in serum and cerebrospinal fluid of the children in VE acute phase and of the children in VE recovery phase were significantly higher compared to the control group(P<0.01). The levels of Gal-9 in serum and cerebrospinal fluid of children in VE convalescent phase are significantly higher than those in acute phase of VE (P<0.01). There was a positive correlation between levels of Gal-9 in cerebrospinal fluid and in serum(r=0.523, P<0.01).4. Nucleated cell counts were significantly increased in CSF of VE acute phase, compared with VE recovery phase(Z=-4.782, P=0.000). About the total protein concentration, there was no significant difference between the two groups(Z=-3.057, P=0.002). There was a weak negative correlation between levels of SP-D in cerebrospinal fluid and nucleated cell counts(rs=-0.393, P=0.002), the same to Gal-9(rs=-0.469, P=0.000).Conclusion:1. SP-D and Gal-9 were involved in the pathogenesis of viral encephalitis, and played an important role.2. SP-D could be an improvement indicator of viral encephalitis. The changes of serum SP-D levels might suggest the changes of SP-D levels in cerebrospinal fluid.3. Gal-9 played an important role in the later stage of viral encephalitis recovery and was very important for immune homeostasis. Gal-9 could be an indicator of complete recovery.4. Nucleated cell count in CSF was meaningful for the diagnosis of viral encephalitis, at the same time, it reflects the severity of immune and inflammatory response to some extent.