| Objective:(1)through large scale clinical studies, study of hepatolenticular degeneration (Wilson’s disease,WD)the common clinical syndromes, and discusses the relationship between clinically relevant factors, reasonable for the further traditional Chinese medicine clinic and related proprietary Chinese medicine development provide a basis(2)Using metabolomics analysis of metabolic markers in patients with typical and its relationship with TCM syndromes and its related clinical factors, in order to provide objective evidence of syndrome differentiation of traditional Chinese medicine, preliminary interpretation of the reason for the exception, and for further study WD pathogenesis and treatment methods based on systems biology.Methods:(1)Base on the integrated literature, design of "WD dialectical types of traditional Chinese medicine questionnaire", collected the patient’s Factors of TCM materials and its syndromes using the Theory of TCM. Establishing databases and using frequency analysis and statistical analysis Chi-square statistical methods, summarizing WD’s common clinical TCM pattern type, and its relationship with clinical syndromes.(2)Collect urine in some patients using MS/GS metabolomics technology to detect changes in metabolites, using frequency analysis and statistical methods of analysis of variance and TCM syndrome types were compared.Results:(2)①In dampness-heat of liver and gallbladders and deficiency of liver-yin and kidney-yin group in the, used GC/MS metabliomic techniques and liver detoxification function related of aromatic family amino,4-hydroxy benzene ketone acid and 4-hydroxy benzene lactic acid no statistics significant (P>0.05),4-hydroxy benzene acetic acid has significantly statistics meaning (P<0.01); and aconitic acid have significantly statistics meaning (P<0.01), both citric acid and succinic acid were no statistics meaning (P> 0.05);②Between dampness-heat of liver and gallbladders and phlegm hot rejection heart group,4-hydroxy benzene ketone acid and 4-hydroxy benzene acetic acid were no statistics meaning (P>0.05),4-hydroxy benzene lactic acid have statistics meaning (P<0.05), and aconitic acid, citric acid, succinic acid were no statistics meaning (P>0.05);③between dampness-heat of liver and gallbladders and spleen deficiency phlegm wettan group,4-hydroxy benzene ketone acid,4-hydroxy benzene acetic acid,4-hydroxy benzene lactic acid were no statistics meaning (P>0.05), aconitic acid have statistics meaning (P<0.05), and both citric acid and succinic acid were no statistics meaning (P>0.05);④between deficiency of liver-yin and kidney-yin and phlegm hot rejection heart group, both 4-hydroxy benzene ketone acid and 4-hydroxy benzene lactic acid were no statistics meaning (P>0.05),4-hydroxy benzene acetic acid have statistics meaning (P<0.05), aconitic acid have statistics meaning (P<0.01), and both citric acid and succinic acid were no statistics meaning (P>0.05);⑤between deficiency of liver-yin and kidney-yin and spleen deficiency phlegm wet group, both 4-hydroxy benzene ketone acid and 4-hydroxy benzene lactic acid were no statistics meaning (P>0.05),4-hydroxy benzene acetic acid have statistics meaning (P<0.01), aconitic acid have statistics meaning (P<0.01), citric acid have statistics meaning (P<0.05), succinic acid were no statistics meaning (P>0.05);⑥ between phlegm hot rejection heart and spleen deficiency phlegm wet group,4-hydroxy benzene ketone acid and 4-hydroxy benzene acetic acid were statistics meaning (P<0.01), succinic acid have statistics meaning (P<0.05), and both citric acid and aconitic acid were no statistics meaning (P>0.05);Conclusion:(1)①here was different traditional Chinese syndroms in all age groups, the length of the course and severity of some may exist in differenr patients, and now dependent on clinical observation has not been able to provide a reliable scientific basis for this distinction. Therefore, still need to actively explore the reasonable support to TCM syndrome typing of scientific method. ② If different age, disease duration and severity, and clinical classification of Western medicine with the common TCM syndromes will be more conducive to improved TCM clinical individualized treatment of hepatolenticular degeneration;(2)①The change of hepatolenticular degeneration of urine metabolism may provide part objective science basis to TCM syndrome; ②hepatolenticular degeneration main to brain lesion and liver injury, metabolomics detection has the advantages of high sensitivity detection, can accurately detect metabolites associated with the injury; ③provide accurate typing of TCM, can help to provide individualized treatment, and for integrated Chinese and Western medicine combines treatment hepatolenticular degeneration building road. |
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