The Research On Relationship Between TCM Syndrome Of Cerebral Infarction And HSP70 Expression

Research Objective: Through detecting HSP70 expression levels after cerebral infarction, combined with traditional chinese medicine theory, analyze the correlation between the expression levels of HSP70 and TCM syndrome types; relationship between HSP70 expression and age, gender, position of cerebral infarction, neurological deficits; relationship between TCM Syndromes and cerebral neurological deficits, position of cerebral infarction.On the one hand provide an objective basis for TCM, on the other hand HSP70 can be regarded as an objective reference index for clinical diagnosis of cerebral infarction and an objective index for assess prognosis of the patients.Methods: According to the diagnostic criteria and exclusion criteria provided by “Practice of Internal Medicine 14 th Edition”, selected 91 patients with cerebral infarction admitted in November 2013 to November 2014, according to the TCM syndrome standards divided into meridian syndrome group and organs syndrome group, at the same time choose met the inclusion criteria 30 healthy people as a control group. All samples of serum HSP70 levels detected by ELISA, Recorded the patients’ age, gender, past medical history, personal history, body mass index(BMI), blood lipid, serum homocysteine(HCY), and CT or MRI and so on.Results:(1) In the 91 cases of cerebral infarction,meridian syndromes group were 72 cases, accounting for 79.12%, the organs syndromes group were 19 cases, accounting for 20.88%.(2) In patients with cerebral infarction group compared with the control group, there was no statistically significant difference in age, gender, smoking history, drinking history etc.(P>0.05), in the aspect of body mass index, history of hypertension, history of diabetes, history of coronary heart disease, the difference was statistically significant(P<0.05); Compared with the meridian syndromes group, Two groups of patients in age, gender, body mass index, smoking history, diabetes history, drinking history difference was statistically significant(P>0.05),in history of coronary heart disease, there was a significant difference(P<0.05), the differences in history of hypertension has significant difference(P<0.01).(3) Compared with the control group, the meridian syndromes group increased TG,LDL,TC,HCY levels, HDL levels decreased, the difference was statistically significant(P<0.05); The organs syndromes group increased TC, TG, LDL, HCY levels, HDL levels decreased, the difference was statistically significant(P<0.01). Compared with the meridian syndromes group, increase the levels of HCY, HDL decreased, the difference was statistically significant(P<0.01); increased TC, TG and LDL levels, the difference was statistically significant(P<0.05).(4) NIHSS score of patients in meridian syndromes group were light group(NIHSS<7) accounted for 22.22%, medium-sized group(7≤NIHSS≤15) accounted for 75%, severe group(NIHSS>15) accounted for 2.78%; while the NIHSS score of patients in organs syndromes group in patients medium-sized((7≤NIHSS≤15)) accounted for 15.79%, severe(NIHSS > 15) accounted for 84.21%. The two groups were compared in neurological impairment score the difference was statistically significant(P<0.05).(5)Comparison of cerebral infarction in patients with NIHSS score for light, medium-sized,heavy, in the expression level of HSP70. The light group compared with medium and heavy group, the difference was statistically significant(P<0.05). The medium group compared with heavy group, the difference was not statistically significant(P>0.05).(6)Compared with the control group, the expression of HSP70 was significantly higher in patients with cerebral infarction group, the differences were statistically significant(P<0.05); Cerebral infarction patients in the two groups were compared, the expression of HSP70 in meridian syndromes group was higher than that of organs syndromes group, the difference was statistically significant(P<0.05).(7)Differences in the level of expression of HSP70 in patients with cerebral infarction in different age groups, with statistical significance(P<0.05), and with the increase of age, the level of Hsp70 decreased gradually. HSP70 levels and age was a significant negative correlation(P<0.01), but no correlation with gender(P=0.407).(8)Between patients with cerebral infarction in different parts of the expression level of HSP70 had no significant difference, no statistical significance(P>0.05).(9)In patients with different TCM syndromes, there was no significant difference between the sites of cerebral infarction(P>0.05).Conclusions:(1)The TCM syndrome types of cerebral infarction is dominated by meridiansyndromes group.(2)Cerebral infarction occurred in elderly male patients, BMI≥28kg/m2, history of hypertension, history of coronary heart disease,dyslipidemia and HCY are the risk factors of cerebral infarction,associated with the syndrome type of TCM, Closely related with the severity of the disease in patients with cerebral infarction. The expression level of HSP70 was related with the syndrome type of TCM, and negatively related to the NIHSS score, the age of the patients. So we think that, for the risk factors of cerebral infarction group, should be treated early and adjust the abnormal index. Determination of HSP70 expression levels as a reference indicator that can be used as an evaluation of such groups tolerance to adverse environmental factors under the stimulation, also can be used as the severity of the patient clinical judgment to assess the prognosis of a biomarker. Provide scientific and objective basis for TCM theory.