Expression Of Liver-intestine Cadherin In Gastric Adenocarcinoma And Its Relationship With Pathology

Objective: Currently, gastric cancer is a common malignant tumor threatening human health. China is one of the countries with the highest morbidity and mortality of gastric cancer, and both the morbidity and mortality were more than twice of the world average values. Adhesion is an important step in the tumor invasion and metastasis, and the first step of tumor invasion and metastasis is the change of adhesion molecules on tumor cells. The intercellular adhesion molecules related to cadherin were closely associated with the invasion and metastasis of tumor cells.As a calcium-dependent transmembrane glycoprotein, the liver-intestine cadherin(LI-cadherin, also called CDH17) is a special member of the cadherin family, and has become a research hotspot attracting much attention in recent years. The study aimed to analyze the function of LI-cadherin in the occurrence and development of gastric adenocarcinoma through detecting its expression in gastric adenocarcinoma.Methods: The resected cancer tissues and corresponding paracancerous normal tissues of 64 gastric adenocarcinoma patients in the General Surgery Department of Harrison International Peace Hospital from January 2014 to November 2014 were collected, as well as their relevant clinical data. A total of 30 cases of intestinal metaplasia tissues and 20 cases of chronic atrophic gastritis(CAG) tissues from the Gastroscope Room of Harrison International Peace Hospital during the same period were collected as the control group. All the patients received no anti-cancer treatments before operation.The LI-cadherin expression and correlation in 64 cases of gastric adenocarcinoma tissues and corresponding paracancerous tissues, 30 cases of intestinal metaplasia tissues and 20 cases of CAG tissues were detected by the avidin-biotin complex(ABC) method. Chi-square test was done by SPSS13 statistical software for statistical analysis.Results:1 The positive expression rates of LI-cadherin in gastric adenocarcinoma, paracancerous normal tissues, intestinal metaplasia and CAG were 53.12%, 0%, 90% and 10%, respectively, with the pairwise comparisons of the cancer tissues with the paracancerous tissues, intestinal metaplasia tissues and CAG tissues all showing significant difference(P<0.05).The expression of LI-cadherin in gastric adenocarcinoma was significantly correlated with the tumor stage, lymph node metastasis, differentiation degree and gastric wall invasion depth, which showed statistical significance(P<0.05).but was not significantly correlated with age and gender(P>0.05).The expression of LI-cadherin in the intestinal metaplasia tissues was correlated with the degree of intestinal metaplasia(P<0.05), but was not correlated was age and gender(P>0.05).2 Based on the WHO pathological classification, the positive expression rates of LI-cadherin in tubular adenocarcinoma, papillary adenocarcinoma, mucinous adenocarcinoma and signet-ring cell carcinoma were 65.38%, 55%, 41.67% and 16.67%, respectively. The pairwise comparisons of the former three showed no significant difference(P>0.05),while the paiwise comparisons of the signet-ring cell carcinoma with the former three all showed significant difference(P<0.05).Based on the Lauren pathological classification, the positive expression rates of LI-cadherin in the intestinal type and diffuse type of gastric cancer were 92.31% and 26.32%, respectively, with the two groups showing statistically significant difference(P<0.05).Conclusion: The expression of LI-cadherin is up-regulated in the intestinal metaplasia and gastric cancer tissues. The abnormal expression of LI-cadherin may be associated with the occurrence and development of intestinal metaplasia and gastric cancer, which means that the expression of LI-cadherin may occur in the early stage of gastric cancer. Therefore, LI-cadherin may become a new marker for the diagnosis of gastric cancer,providing clinical guidance for the early diagnosis of gastric cancer.