Quantitative Detection Of Neutrophil CD64 And CRP In The Diagnosis And Treatment Of Chronic Prostatitis

Objective:Detecting the expression of neutrophils CD64 and levels of blood C-reactive protein(CRP) by flow cytometry in prostatic fluid, to assess its value in the diagnosis of chronic prostatitis, and provide a reliable basis for the clinical diagnosis of chronic prostatitis.Methods:1. Sterile specimens prostatic fluid for bacterial culture, Chlamydia trachomatis(CT), Ureaplasma urealyticum(UU)detection and prostate fluid routine examination. 2. Quantitative analysis neutrophil CD64 expression levels and leukocyte count absolute in the prostatic fluid by flow cytometry,and detecting CRP over the same period. Combining detection CD64 and CRP, dynamic observation the test results, and determine the condition and efficacy. 3. Dynamic observation CD64 and CRP test results, and analysis and monitoring the efficacy of significance and value within the disease progression as CD64 and CRP combination. 4. Guiding patients to fill Chronic Prostatitis Symptom Index(NIH-CPSI)scale, than study the relations within CD64 expression levels, CRP levels and NIH-CPSI score to explore the relevance.Result:1. The experimental group were 130 cases for bacterial culture, Staphylococcus epidermidis, Staphylococcus epidermidis, Staphylococcus aureus, Warner aureus, Enterococcus faecalis, Proteus mirabilis, Staphylococcus haemolyticus, intermediate Staphylococcus aureus xylose, man-aureus, Escherichia coli, Chlamydia trachomatis, Ureaplasma urealyticum were detected in prostatic fluid of chronic bacterial prostatitis patients.2. The results of microscopic examination and flow cytometry showed prostatitis group leukocyte counts were significantly higher than the healthy group(P <0. 01). But there was no significant difference in two counting methods. Then compared the count results between the experimental group and the healthy group. The results showed that the leukocyte counts in bacterial infection group and CT / UU groupwere significantly higher than the healthy group(P <0 01); Flow cytometry results showed thatthe bacterial infection group was also significantly higher than the CT / UU group(P <0. 05). 3. CPR levels for the experimental group was(5.12 ± 3.56) mg / L, the normal group was(2.33 ± 0.75) mg / L. CRP level decreased one week after treatment, and has been close to normal surrounded by weeks. There was a positive correlation between the CPR levels before and after treatment and CPSI score. 4. CD64 expression levels in healthy control group was 966. 214, CP group was 2183.693, P <0. 01.There was a significant difference as CD64 expression of CP group was significantly higher than the control group.There was a positive correlation between the CD64 expression levels before and after treatment and CPSI score. 5. The CD64 expression levels before and after treatment with CRP and CPSI score correlation analysis, there was a positive correlation.Conclusion:1.This study measured peripheral blood serum C-reactive protein and neutrophil CD64 expression in prostatic fluid by flow cytometry, as early diagnostic marker of infect ious chronic prostatitis patients. 2. To clear the neutrophil CD64 expression in prostatic fluid and CRP levels. At the same time, NIH-CPSI score was recorded, and correlation analysis, will contribute to the observe clinic judgment and drug efficacy, Thus providing valuable experimental basis for clinical diagnosis and treatment of prostatitis.