The Mechanism Study Of MiRNA-206 Participating In Metabolic Abnormalities In Patients With Hyperthyroidism

Objective: To investigate serum mi R-206 expression and the mechanism of mi R-206 participating abnormal lipids metabolism in hyperthyroidism patients.Methods: The serum specimen of 15 hyperthyroidism patients and 12 healthy people were respectively obtained from Endocrinology Clinic and Health Examination Center of the 82 nd Hospital according to inclusion and exclusion criteria, from October, 2011 to December, 2012. The mi R-206 expression level of serum specimen was assessed through q RT-PCR. The correlation between mi R-206 expression level and FT3, FT4, TSH level was analysisd by using linear regression analysis. In addition,The mi R-206 expression level was also detected by q RT-PCR and TG level was detected by enzymatic colorimetric method and stained by oil red O in Hep G2 cells which were cultured in medium with T3. The level of TG was also identified by enzymatic colorimetric method after downregulation/overexpression of mi R-206 in Hep G2 cells. The TG level was detected in cells which were cultured with T3 after overexpression of mi R-206 in Hep G2 cells.Results: 1. Compared with the healthy people, the expression of mi R-206 Is downregulated significantly in serum of hyperthyroidism patients by q RT-PCR(p<0.05). Additionally, FT3 concentration and mi R-206 level in the serum specimen are significantly negatively correlated(r= ﹣ 0.408, p=0.035), as well as FT4 concentration and mi R-206 level(r=﹣0.451, p=0.018) while TSH concentration and mi R-206 level are significantly positively correlated(r=0.445, p=0.020). 2. Mi R-206 is downregulated apparently in Hep G2 cells after cells were cultured 24 h, 36 h with T3(p<0.05). 3. TG level is reduced obviously in Hep G2 cells after cells were cultured24 h with T3(p<0.01). 4. TG level is reduced/increased obviously after mi R-206 weredownregulated/overexpressed in Hep G2 cells(p<0.05). 5. TG level is increased obviously in Hep G2 cells which were cultured with T3 after overexpression of mi R-206, which indicate that overexpression of mi R-206 blocks T3 decreasing TG level in Hep G2 cells(p<0.05).Conclusion: 1. Mir-206 is significantly down-regulated in serum of hyperthyroidism patients. 2. Mi R-206 level specimen and TH concentration in the serum are significantly negatively correlated. 3. Mir-206 participates in the regulation of TG in Hep G2 cells. 4. Mir-206 may mediates TH regulating TG metabolism.