| Objective:This study use matched case-control method to implore the effect of high Serum Luteinizing Hormone level during mild ovarian stimulation protocol on embryo quality and IVF outcomes.Methods:This was a retrospective, observational study including a total of180patients underwent IVF/ICSI from October2012to October2013in the Assisted Reproductive Center of Shanghai Ninth People’s Hospital.90patients with high LH level in follicular phase was selected as case group, while90patients as controls in minimal stimulation cycles were applied by pairs and matched by criteria such as age, infertility background and stimulation protocol. According to ovarian reserve and infertility background, the study population was further divided into three groups:PCOS group, SOF group and Normal group. In this study, the rate of oocytes retrieved, the rate of high valid embryos, cancellation rate, implantation rate, chemical pregnancy rate and clinical pregnancy rate between high LH level case groups and control groups in three groups were specifically evaluated.Results:There are no significant difference between High LH group and control group on the number of oocytes retrieved, although serum LH level in High LH group was significantly higher than that in control group, P>0.05.The study population was divided into three groups according to ovarian reserve and infertility background. Group A、B and C are, respectively, normal ovarian reserve group, PCOS group and Diminished ovarian reserve group. In Normal ovarian reserve group, the number of oocytes retrieved (2.70±1.67vs.3.0±1.85), fertilization rate (74.2%vs.73.9%), cleavage rate (97.8%vs.98.0%), High quality embryo rate (97.8%vs.98.0%) between case group and control group were not significantly different, P>0.05. After FET cycle, the implantation rate (36.8%vs.40.7%)、chemical pregnancy rate (64.2%vs.64.7%)and clinical pregnancy rate(50%vs.52.9%)in case group was not significantly different with that in control group, P>0.05.Within PCOS group, the number of oocytes retrieved (6.86±3.35vs.6.67±3.40), fertilization rate (74.2%vs.73.9%), MII embryo rate (90.7%vs.87.6%), cleavage rate (97.1%vs.96.3%), High quality embryo rate (63.6%vs.68.3%) between case group and control group were not significantly different, P>0.05. After FET, our data shown that the implantation rate (33.3vs.33.6%)、chemical pregnancy rate (61.5%vs.61.9%)and clinical pregnancy rate (53.8%vs.57.1%)in case group was not significantly different with that in control group, P>0.05.While, according to the data gathered in diminished ovarian reserve group, there were no statistical differences among case and control group in infertility rate (97.91%vs.94.29%), cleavage rate (42.47%vs.49.31%) and average high quality embryo number (1.29±0.49vs.1.24±0.53), P>0.05. In FET cycle, implantation rate (36.8%vs.33.3%) showed no significant between-group difference, P>0.05. However, the chemical pregnancy rate (46.7%vs.40.0%) and clinical pregnancy rate (40%vs.35%) have significantly between group differences between two groups, presumably due to the small number of FET cases.Conclusion:Under the premise of unselective all embryos cryopreservation, the result of ovarian stimulation outcomes and pregnancy outcomes indicate that high LH case group and control group showed no differences in embryo quality, which indicating that High LH level does not necessarily impair oocyte development and maturation, as well as embryo development The outcomes of FET using these embryos have no obvious statistical significance. |
PDF Full Text Request