| Object： To determine whether chronic alcoholism alters theexpression levels of VIP and SP in the cochlea of chronic alcoholism rats.Methods：we measured their expression levels in30Sprague–Dawleyrats, in which we created models of different degrees of chronic alcoholism.We investigated the presence of the mRNA of VIP and SP in the cochlea ofchronic alcoholism rats and controls using the reversetranscription-polymerase chain reaction (RT-PCR) method. We alsoevaluated the local distribution of VIP and SP cells byimmunohistochemistry. We investigated the presence of proteins of VIP-receptor1（VIPR1） and SP-receptor（S PR） in poisoned rats andcontrols by using western blot.Results：We found that the levels of VIP and SP were downregulated inthe chronic alcoholism groups compared to the controls group. Thedifferences in some expression levels were significant between chronicalcoholism rats and control rats. Moreover, at different degrees of alcoholpoisoning in rats, the contents of VIP and SP differed. Decreased levels ofVIP and SP were detected in the deep chronic alcoholism group compared to the group with low-degree poisoning.Conclusion：These results suggest that VIP and SP play an importantrole in the auditory function in rats with chronic alcoholism. Chronicalcoholism may cause a peptide hormone secretion imbalance in the auditorysystem, eventually leading to hearing loss. |
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