APP And Aβ Protein Expression In Mice Neurons With Chronic Alcoholism

IntroductionChina is a country with a large population and the aging process is very fast. China is a long history,and diverse cultures co-exist wine in the country.Because of the particular social and historical reasons,alcoholism frequently is the case.Chronic alcoholism is characterized by addiction,as is often repeated in drinking on the ethanol tolerance,when it occurs when alcohol withdrawal(withdrawal) symptoms of the nerve.Nervous system caused by chronic alcoholism and serious damage to other organs,including nervous system damage to the most serious.Practice in forensic science,and sometimes encounter cases of alcoholism are often associated with traffic accidents,major organ dysfunction and other related organizations.Alzheimer's disease(AD) is a progressive neurodegenerative disease,manifested as a comprehensive cognitive impairment.The main histopathological changes is derived from amyloid precursor protein(APP) ofβ-amyloid peptide(Aβ) consisting of amyloid plaque and excessive or abnormal phosphorylation of Tau protein composition neurofibrillary tangles.The incidence of Alzheimer's disease are subject to many factors,including environmental factors,poor dietary habits,drug types,such as poisoning.Chronic alcoholism lead to brain APP and Aβprotein in order to trigger an increase in the incidence of Alzheimer's disease,is still a lack of systematic theoretical and experimental studies.Neuron-specific enolase(NSE) is a sign of nerve cell protein,the existence of specificity in neurons and neuroendocrine cells in the brain of all soluble protein accounted for 1.5%of neuronal damage or NSE into the blood after necrosis.In this study,mice in the establishment of animal models of chronic alcoholism, based on the application of histopathological and immunohistochemical techniques, research in mice after chronic alcoholism and brain nerve cells in the expression of APP and Aβ,and the application of SPSS statistical software of the observed experimental results were statistically analyzed to explore neurons in chronic alcoholism APP and Aβ,as well as the content changes in the incidence of Alzheimer's disease and the causal link between them.Materials and Methods1.Establishment of animal model and packet42 9-week-old Kunming mice were randomly divided into 30-day group,the 60-day groups was divided into 10%alcohol group,20%alcohol group and the control group,of which five of the control group,10%ethanol group 8,and 20%alcohol group 8.The experimental group will be anhydrous ethanol(HPLC pure) in drinking water to match the concentration of 10%,20%alcohol solution as the drinking water of mice in the control group used in place of drinking water.After the end of the experiment, animals were killed directly decapitation,rapid extraction of mouse brain tissue,4% paraformaldehyde/PBS pH7.4 fixed,paraffin-embedded,producer.2.Methods(1) HE staining to observe the application of the morphological characteristics of organizations.(2) the use of Nissl staining of nerve cells circumstances Nissl bodies.(3) Application of neuron-specific enolase staining patterns characteristic of neurons.(4) immunohistochemical staining APP and Aβin the brain tissues.(5) the use of Motic Images Advanced 3.2 image analysis system to calculate immunohistochemical staining positive cells and expression of the area.(6) the application of SPSS statistical software for statistical analysis of experimental dataResult(1) The experimental group of mice significantly the performance of chronic poisoning,and decreased body weight. (2) HE staining of chronic alcoholism see the mice brain edema,nerve cells in the disappearance of Nissl bodies or margination,nerve cells around the gap widened.(3) neuron-specific enolase immunohistochemistry see increase in enzyme protein content.(4) the control group shows that a small number of APP positive immunohistochemical staining of nerve cells,a product of the distribution of staining in the cell cytoplasm,showing brown yellow.Experimental group,APP staining positive cells markedly increased expression of different concentrations and different time periods of different intensity of positive expression.Experimental group,nerve cells showed a visible product of Aβ-positive staining,and high concentrations of prolonged treatment group the number of positive cells markedly increased expression.Using Motic Image Advanced 3.2 image analysis system to calculate the average integrated optical density,and the use of SPSS software for statistical analysis and found that the experimental group and the control group there was significant difference.Conclusion(1) Chronic alcoholism can cause brain cells of mice degeneration.(2) Chronic alcoholism can cause brain cells of mice neuron-specific enolase increase in content and distribution of abnormalities.(3) Chronic alcoholism can cause brain cells of mice produce Aβprotein,and APP protein expression was significantly enhanced.(4) Chronic alcoholism may be induced by Alzheimer's disease,one of the relevant factors.