The Association Between FoxO4and Decidual Actue Atherosclerosis In Preeclampsia

Objective:The aim of this paper is to explore a possible association between FoxO4expression and the formation of decidual acute atherosclerosis in preeclampsia。Meanwhile, we also investgate a possibility of FoxO4may accumulate the oxidative stress by regulating the formation of decidual acute atherosclerosis.Methods:Serum and decidua samples were obtained from25patients with preeclampsia and15normal pregnant women, and all the patients terminate the pregnancy by caesarean.40decidual tissues were routinely stained with haematoxylin and eosin (H&E). And the Immunohistochemistry was used to locate phosphorylates FoxO4(P-FoxO4). The serum was analysised by ELISA for8-isoprostane concentration. Meanwhile the decidua tissue was analysised by Western Blot to investigate the concentration of8-isoprostane, FoxO4and P-FoxO4. And the SPSS software was used to analysis the results.Results:Decidual acute atherosis was present in40%of the preeclamptic patients (10/25), however none of the normal pregnancies possess any decidual acute atherosis. We separated the patients to3grops based on the H&E results:PE+Aa, PE-Aa and NP. The ELISA showed the difference plasma level of8-isoprostane between the3groups (P<0.01):NP group was155.6±11.0pg/ml, PE-Aa group was250.8±30.1pg/ml, and the PE+Aa group was338.1±27.5pg/ml. The decidual8-isoprostane protein concentration was increased between3 groups (P<0.01):NP group was0.31±0.07, PE-Aa group was0.41±0.09, and the PE+Aa group was0.53±0.07. The decidual FoxO4protein concentration was increased between3groups (P<0.01):NP group was0.33±0.04, PE-Aa group was0.48±0.07, and the PE+Aa group was0.63±0.10. The decidual P-FoxO4protein concentration was increased between3groups (P<0.01):NP group was0.26±0.04, PE-Aa group was0.40±0.06, and the PE+Aa group was0.50±0.09. Decidual concentration of FoxO4and P-FoxO4are positively correlated with decidual and plasma level of8-isoprostane.Immunostaining demonstrate that abundance P-FoxO4aggregating in cell nucleus in PE+Aa group patient, while less or even no such observation showed in group PE-Aa or NP.Conclusions:The increasing of FoxO4and P-FoxO4may contribute to the formation of "Decidual acute atherosis", and hence increasing the level of8-isoprostane. There is a possibility that the increasing of FoxO4in decidual may augmente oxidative stress in the maternal systemic circulation.