The Investigation Of The Correlation Between8-isoprostane, ROS And Preeclampsia Progress

Preeclampsia(PE) is strongly to human pregnancy deaths.Although the mechanismsunderlying the pathogenesis are not fully understood, bring certain difficulty to preventionand treatment. Although with the level of medical technology continues to improve,but atpresent it still PE is second leading cause of maternal mortality, perinatal children also leda major cause of morbidity and mortality.Objective：1.To explore the8-iso-prostaglandin (8-isoprostane)，malondialdehyde (MDA)，superoxide dismutase (SOD)，reactive oxygen species (ROS) and other oxidative stressproducts, ultra-sensitivity C-reactive protein (hs-CRP)，interleukin-6(IL-6)，tumornecrosis (TNF-α) and other inflammatory markers and lipid levels to patients withpreeclampsia, and to explore oxidative stress.Expression and significance of inflammatorymechanisms and lipid of preeclampsia.2.To analyze the correlation between lipid peroxidation and lipid profiles.Inflammationand to further investigate the correlation between lipid peroxidation and providingpreeclampsia data for clinical diagnosis and treatment.Methods：The100patients were selected according to the diagnostic standard forpreeclampsia,including60cases of mild preeclampsia, severe preeclampsia40cases, while60women without pregnancy complications as control group. Maternal peripheral bloodspecimens were collected for8-isoprostane，MDA，SOD，ROS and hs-CRP，IL-6，TNF-α levels, and lipid profiles were assessed at the center of blood test in our hospital.All the data were analyzed by SPSS11.5software.Result：1.Case group8-isoprostane (164.86±14.78) ug/L and ROS (19.81±8.67) U/L level was significantly higher (156.32±13.86ug/L；11.32±6.86U/L), the difference wasstatistically significant significance (p<0.05), and severe preeclampsia8-isoprostane(166.42±11.42) ug/L and ROS (21.45±9.85) U/L levels were significantly higher inpatients with mild preeclampsia (161.63±13.64ug/L；15.65±9.67U/L), the differencewas statistically significant (p<0.05), SOD in the case group (89.78±11.42) U/L wassignificantly lower than the control group (105.64±12.86) U/L, the difference wasstatistically significant (p <0.05), and severe preeclampsia SOD (86.45±10.89) U/Llevels were significantly lower in patients with mild preeclampsia (91.22±13.74) U/L,the difference was significant the statistical significance (p <0.05).MDA levels were higherin the case group, but the difference was not statistically significant (p>0.05), and patientswith severe preeclampsia MDA levels were higher in patients with mild preeclampsia, butthe difference was not statistically significant (p>0.05).2.Case group IL-6(27.74±4.90) pg/ml， hs-CRP (2.23±1.31) mg/L and TNF-α(19.78±2.75) pg/ml was significantly higher (13.43±2.56pg/ml;1.65±0.56mg/L；8.64±1.43pg/ml), the difference was statistically significant (p <0.05), and severepreeclampsia IL-6(29.53±4.52) pg/ml, hs-CRP (2.46±0.86) mg/L, and TNF-α (21.45±3.89) pg/ml levels were significantly higher in patients with mild preeclampsia (23.42±3.66pg/ml；1.83±0.45mg/L;14.32±2.78U/L), the difference was statisticallysignificant (p <0.05).3．Cases in group TC (6.65±1.64mmol/L), TG (3.64±1.85mmol/L) and LDL-C (3.18±0.57mmol/L) was significantly higher than that of the control group (5.63±1.75mmol/L;2.23±0.67mmol/L;2.53±0.75mmol/L), with statistical significance the difference (P <0.05), and severepreeclampsia patients with TC (7.23±1.74mmol/L), TG (3.96±1.42mmol/L) and LDL-C (3.55±0.64mmol/L) was significantly higher than that in mild preeclampsia patients (6.42±1.43mmol/L;3.13±0.86mmol/L;2.85±0.75mmol/L, the difference was statistically significant (P <0.05).4．A significant positive correlation between8-isoprostane level and IL-6, hs-CRP, TNF-α ofpatients with preeclampsia serum alpha (P <0.01), ROS level and IL-6, hs-CRP, TNF-α positively (P<0.05). A significant positive correlation between the plasma8-isoprostane level in patients with preeclampsia and TG (p<0.01), the level of ROS was positively correlated with TG (P <0.05).Conclusion:1. Preeclampsia plasma8-isoprostane and ROS levels were significantly higher inpatients with severe preeclampsia8-isoprostane and ROS significantly higher in patients with mild preeclampsia, indicating the presence of preeclampsia is not onlyexcessive oxidative stress, and increased with disease progression.2.Preeclampsia plasma hs-CRP，IL-6and TNF-α levels were significantly higher inpatients with severe preeclampsia IL-6，hs-CRP and TNF-α was significantly higher thanmild preeclampsia, indicate preeclampsia exist not only excessive inflammatory response,and increased with disease progression.3. Preeclampsia serum TC，TG and LDL-C levels were significantly higher in patientswith severe preeclampsia TC, TG and LDL-C significantly higher than the mildpreeclampsia, indicating the presence of preeclampsia is not only excessive fat metabolismand increased with disease progression.4.Preeclampsia seru8-isoprostane and ROS levels and IL-6， hs-CRP，TNF-αshowed a significant positive correlation and preeclampsia plasma8-isoprostane and ROSlevels with TG showed a significant positive correlation, indicating that oxidative stress,inflammation and lipid abnormalities may play a role in preeclampsia synergisticintervention oxidative stress, inflammation and lipid abnormalities may help to control theoccurrence and development of preeclampsia.