Effects Of Exhausted Swimming Styles On The Expression Of NF-κB And CAMP In Rat NAc

Objective: To observe the expression of NF-κB and cAMP in Rat NAc of adult rats after exhausted swimming, and investigate the mechanism of NAc after exhausted swimming. Providing theoretical evidences for exercise training,and offering new ideas for exercise induced disease prevention and sport treatment.Methods: Eighty SD rats were divided into the control group (n=10) and exhausted group (n=70) after adaptive training. The fresh cerebral tissues were divided on ice water bath while the paraform-perfused brains were obtained at 0h,4h,8h,12h,16h,20h,24h after exhausted swimming for a week. The former cerebral tissues were detected by Western Blot and ELISA in order to investigate the changes of NF-κB and cAMP in NAc, however, the later perfused brains be done with frozen section, immunohistochemical staining, then the change of expression of NF-κB were observed. The same to control group.Results: (1). Western Blot showed, the expression of NF-κB was extremely lower in control group, but increased higher after exercise. In NAc, the expression of NF-κB increased to the higher level at 8h after exhausted swimming,then,retainned high level for a monment, peaked at 16h, then descended gradually. (2). Compared with control group, the number of NF-κB+ neurons had no significant difference at 0h. Several NF-κB+ neurons of control group distributed in the NAc dispersively while the exhausted groups expressed significantly higher after 4h, and increased to the higher level at 8h, then, retained the high level, peaked at 16h, then descended dramatically.(3). The expression of cAMP was extremely lower in control group, but transiently increased at 0h after exhausted exercise, then increased to the top at 8h ,retained high level to 16h,and descended gradually.(4). NF-κB is the same as cAMP in time course.Conclusion: (1). NF-κB and cAMP can be activated effectively in NAc after exhausted exercise. (2).the tendencys of change of NF-κB and cAMP in NAc after exhausted exercise are similar, It is suggested that they have synergistic effect. (3). NF-κB and cAMP signals passways may be offering new therapy means for relative-NAc-disease,and providing theoretical evidences for exercise training.