Study Of The FoxA1Expression In Gastric Cancer And Its Relationship With Clinicalpathological Parameters

BACKGROUNDCancer of the stomach (gastric cancer,GC) is one of the most common malignancies in our country. Because of Lacking of sensitive and specific early diagnostic molecular marker, patients with symptoms are always in the advanced stage, which leads to a poor survival rate. As the improvement of the diagnosis technology and medical technology, its treatment effect and the clinical prognosis are still in low level. Tumor recurrence and metastasis is the leading cause of the high mortality with gastric cancer, and is also the key factors of the poor clinical outcome. Therefore, it’s of vital significances to further investigate the mechanisms of carcinogenesis, progression, invasion and metastasis of gastric cancers.Fox contain an about110amino acids DNA-binding domain or FOX/winged helix domain highly conserved. As the first forkhead box protein found in mammals, hepatocyte nuclear factor3alpha(HNF3a) was later named FoxAl. Expressed in the tissue of adult endoderm (such as the liver, lung, pancreas, etc.), mesoderm (e.g., kidney, uterus, breast, etc.) and ectoderm (such as the brain, the olfactory epithelium, etc.),FoxAl is required for individual growth and development, and plays different role in multiple organs, and a close relationship with clinical occurrence and progress of a wide variety of tumors. However for lest of our knowledge, there is no publicized literature describe the relationship between the FoxA1and the clinic pathological features in gastric cancer patients.OBJECTIVEInvestigate the expression of FoxAl in gastric cancer and analyze the relationship between FoxA1expression and clinic pathological features of gastric cancer.METHODS1. The expression of FoxAl mRNA and protein in34fresh specimens of gastric cancer tissues and adjacent non-cancer specimens was detected by qRT-PCR and western blot.2. The expression of FoxAl in104specimens of gastric cancer tissues and adjacent non-cancer specimens was detected by immunohistochemistry (IHC). The relationship between FoxAl expression and clinicopathological features, prognosis was statistically analyzed.RESULTS1. The expression of FoxA1in gastric cancer tissues was significantly lower than that in adjacent non-cancer gastric mucosa tissues (P＜0.01).2. Expression of FoxA1was statistically significantly associated with the depth of invasion, TNM stage, the grade of tumor differentiation, numbers of lymph node metastasis(P<0.01).,but not with the patients age and sex(P>0.05).3. In univariate analysis, lower expression FoxAl was associated with5-year survival rate. Moreover, the impact of FoxAl expression on5-year survival rate was statistically significant (P<0.01).. In Cox multivariate analysis, expression of FoxAl immunostaining was found to be independent prognostic factors(P<0.01).. CONCLUSIONLower expression of FoxAl is not only closely related with invasion and metastasis of gastric cancer, but also an independent prognostic factor and might be regarded as a factor of poor outcome in gastric cancer.