The Study Of Electric Acupuncture Intervention On AVP Antagonize A β1-40in Hippocampal CA1Region Of VD Rat

Objective:Observing the VD rat models after ischemia-reperfusion, and the hippocampus CA1area AVP and Aβ1-40metabolic process, after the treatment groups compared with model group, to explore the curative intervention, the rat hippocampus of AVP and Aβ1-40secretion in neural modulation of synaptic transmission efficiency, the neurotoxice effects. By means of EA1group1(VD model+EA), EA2groups (VD model rats plus injecting of AVP receptor blockers+EA) after treatment of dementia rat hippocampal CA1area of AVP, Aβ1-40protein contrast observation, combined with Morris water maze behavior test, as well as Western Blot method, to test results, to observe the VD rats hippocampus CA1of AVP, Aβ1-40different protein content, and to explore the electric acupuncture to intervene in the rat hippocampal CA1area of AVP and Aβ1-40protein expression quantity change mechanism.Methods:The70SD rats were randomly divided into six groups, which are control group, model group, blockers, EA1group (VD model+EA), EA2group (VD model rats plus injecting of AVP receptor blockers+EA), and western medicine group. Except randomly8only as control group, the experimental rats with modified Pulsinelli.4-VO prepared dementia model. Select qualified after VD rats, selected from22VD randomly CA1area of rats injected AVP receptor blockers. Comparing the change of model rats laboratory tests before and after acupuncture and behavioral changes, and using Western Blot detection method, comparison after treatment, each group of VD rat hippocampus CA1of AVP, A β1-40different protein content, and application of statistical hypothesis test in combination with the corresponding index change, and to explore the electric acupuncture to intervene in the rat hippocampal CA1area of AVP and Aβ1-40protein expression quantity change mechanism.Results:1, Morris water maze results:EA1group, the western medicine group compared with model group, EA2groups with blockers comparison, the average escape latency decrease, for the first time across time, across the original platform within120s increase (P<0.05); Blockers group compared with model group, to escape the incubation period for the first time, lengthen across time, across the original platform within120s less (P<0.05); EA1group compared with the western medicine group, the average escape latency decrease, for the first time across time, across the original platform within120s increase (P<0.05).2, Western Blot method test results:EA1group, the western medicine group compared with model group, EA2groups with blockers comparison, AVP protein content increased, Aβ1-40protein content decreased (P<0.05); Model group compared with control, AVP protein content decreased, Aβ1-40protein content increased (P<0.05); EA1group compared with the western medicine group, EA1group AVP protein content is more, Aβ1-40less protein content (P<0.05).Conclusion:Using modified Pulsinelli.4-VO blocking method, can better simulate the pathogenesis of VD, the rats, which were injecting of AVP receptor blockers into hippocampal CA1area, could reduce the protein content of AVP, and the brain Aβ1-40protein content increased; Electric acupuncture in improving the testing results of the VD rats behavior, the effect is obvious, is superior to western medicine group, the mechanism may be related to improvement of rat hippocampus AVP, Aβ1-40in neural modulation of synaptic transmission efficiency and the nerve toxicity effect.