Experimental Study Of Reaction And Effection Of PAR And IL-8in Prostate Cancer Cells

Objective:Observation of expression and function of protease activated receptor PAR1,2,4and interleukin-8in different prostate cancer cells, explore the molecular pathological mechanism of prostate cancer。Methods:1、cultivation of prostate epithelial cell lines HPrEpiC, androgen dependent prostate cell lines LNcap, androgen independence prostate cancer cell lines DU145, PC-3cell lines, and detection the expression of PAR1, PAR2, PAR4, IL-8gene and protein;2、grouping cells into four groups depend on joining different agonistS。After join PAR1(20umol/L), PAR2(150umol/L), PAR4agonists (300umol/L),cells with be determined by MTT method to detect prostate cancer cell proliferation ability in12h、24h、48h;3、grouping cells, join PAR1(20umol/L), PAR2(150umol/L), PAR4(300umol/L) agonists, then use Real-time PCR and Western blotting technology to detect the expression of PAR1, PAR2, PAR4, and IL-8。Results:1、prostate cancer cells (LNcap cells, PC-3, DU145cells) and prostate epithelial cells in HPrEpiC have PAR1, PAR2, PAR4, and IL-8gene and protein expression; The expression of PAR IL-8gene and protein in prostate cancer cells is higher than the prostate epithelial cells; The expression of PAR and IL-8gene and protein in androgen independence prostate cancer cells than in androgen dependence prostate cancer; 2、MTT results show:in three kinds of prostate cancer cells (LNcap cells, PC-3, DU145cell), the activation of PAR1,2,4can enhance prostate cancer cells’ proliferation (P<0.05); And PAR1, PAR2, PAR4have the same effects in enhancing the proliferation (P>0.05); In same condition, three kind of prostate cancer cells’(LNcap,PC-3,DU145)proliferation ability have no significant difference (P>0.05);3、Real-time, western blot test results show:PAR1, PAR2, PAR4agonist can activate the corresponding PAR receptors in the prostate cancer cells (LNcap cells, PC3, DU145cells) and prostate epithelial cells HPrEpiC; In PAR activated prostate cancer cells, the IL-8expression increased significantly, but the change of IL-8in the prostate epithelial cells HPrEpiC is not obvious (P<0.05); The expression of PAR and IL-8in androgen independence prostate cancer (DU145, PC-3) is higher than androgen dependent prostate cancer (LNcap)。Conclusions:1、prostate cancer cells (LNcap cells, PC-3, DU145cells) and prostate epithelial cells (HPrEpiC) have PAR1, PAR2, PAR4and IL-8gene and protein expression; The expression of PAR and IL-8in prostate cancer cells is higher than the prostate epithelial cells; The expression of PAR and IL-8in androgen-independence prtostate cells (PC-3, DU145cells) is higher than androgen-dependent prostate cancer (LNcap);2、The content of IL-8and prostate cancer (LNcap cells, PC-3, DU145cell) cell proliferation ability has a positive correlation;3、The rise of protease activated receptor in prostate cancer cells (LNcap、PC-3、 DU145)cells enhance the proliferation; In same proliferation, the expression of IL-8in androgen-independence prtostate cells (PC-3, DU145cells) is higher than androgen-dependent prostate cancer (LNcap);4、The protease activated receptor may be a novel targets for drug treatment of prostate cancer。...