Protease Activated Receptor 1, Protease Activated Receptor 2, Expression In Her Lesions With Psoriasis Vulgaris, And Its Relationship With Blood Vessel Growth

Objective: Psoriasis is a common chronic recurrent skin disease characterized by erythema, papules,scales. Dermal papilla anomalies appear earlier in the histopathologic changes of psoriasis,followed by the transition of inflammatory cells and epidermal hyperplasia and prosoplasia.Vascular anomalies not only launch the whole of the pathological process of psoriasis, but as a result of the vascular hyperplasia, circuity and the increase of permeability, a large number of immune cells and its regulatory factors exude to the outside of the blood vessels, and stimulate the secretion of cytokines and the proliferation of malpighian cells, and the feedbacked stimulation promotes the change of the blood vessels. Angiogenesis is a complex process, and there are many factors involved. Protease activated receptor(PAR) is the family of G protein-coupled receptors, which were discovered gradually in the twentieth century. In the family, PAR1 distributes most widely, and it’s also the most important. PAR1 can promote cell proliferation and angiogenesis. Vascular endothelial growth factor(VEGF) is an angiogenic factor and it’s very important. It can not only increase the permeability of the blood vessels, but can promote endothelial cell division and proliferation, and make angiogenesis. With the further study of PAR2, people find that PAR-2 is closely related to VEGF and could promote angiogenesis.Immunohistochemical method was used to examine the patient’s PAR1 and PAR2 expression in advanced and stable periods.. We used CD34 to mark vascular endothelial cells to count microvessel density of psoriatic lesions. By studying the expression of PAR1、PAR2 in psoriasis vulgaris, and its relationship with angiogenesis, we could explore their roles in the pathogenesis of psoriasis vulgaris.Methods:32 cases with psoriasis vulgaris were taken from the patients of the third people’s hospital of Botou city in Hebei Province from November 2013 to April 2014. All of the patients had typical clinical manifestations,and they were confirmed for psoriasis by the histopathologic. There were 19 male cases and 13 female cases. They were aged from10 to 65 years old and their average age is 33.9. Of the 32 cases 23 were in active phase and 9 in stationary phase. All patients did not completely or partly receive treatments of some drugs such as glucocorticoid and vitamin A acid drugs and immunosuppressants at least two months, and had no other system diseases. Immunohistochemical SP method was used to detect the expression level Of PAR1, PAR2 and CD34 in patients with lesions. Compared with 10 cases of healthy facial skin from the cosmetic surgery department, no skin diseases and systemic diseases were found. The 10 cases included 7 male ones and 3 female ones from 20 to 53 years old. Their average age was 35.6. There were no statistic significance in the difference of age and sex between the two groups. We tested the experimental results using SPSS16.0 statistical software. We designed t test comparing measurement data between groups, and used c2 test comparing the count data between groups, and used the mean +/- standard deviation(c±s)representing measurement data. We used spearman rank correlation analysis in Correlation analysis. Significant boundaries was P< 0.05.Results:1 PAR1 expressed mainly in the cutin cell membrane of epidermis with psoriasis vulgaris from basal layer to stratum spinosum. In the 32 cases,there were 5 cases of negative expression and 27 cases of positive expression. In 10 cases of normal contrast group, there were 5 negative cases and 5 positive cases and they expressed mainly in the basal layer. According to the experimental results, the positive expression of PAR1 in patients with psoriasis was higher than the normal compared group, and there was a very significant difference(P<0.05). The expression of active stage was higher than stationary phase and the difference was statistically significant(P<0.05).2 PAR2 expressed mainly in the cutin cell cytoplasm and cell membrane of epidermal granular layer and stratum spinosum with psoriasis vulgaris. In the 32 cases of with psoriasis vulgaris, there were 6 cases of negative expression and 26 cases of positive expression. There were 6 negative cases and 4 positive cases in 10 cases of normal contrast group, and they expressed mainly in epidermal stratum spinosum. According to the experimental results,positive expression of PAR2 in patients with psoriasis was higher than normal contrast group. The expression of active stage with psoriasis vulgaris was higher than stationary phase and the difference was statistically significant(P< 0.01).3 CD34 expressed mainly in cytoplasm superficial dermal microvascular endothelial cells at psoriasis group and normal contrast group. But MVD in the psoriasis vulgaris group was higher than normal contrast group. There was a very significant difference(P<0.01). MVD of active stage with psoriasis vulgaris was higher than stationary phase, and the difference was statistically significant(P<0.01).4 The expression of PAR1 and the expression of PAR2 in psoriasis vulgaris were positively correlated. The coefficient correlation rs=0.626.The difference was statistically significant(P<0.01).5 The expression of PAR1 and PAR2 was positively correlated with microvascular density in the lesion with psoriasis vulgaris(rs=0.694,rs=0.712,P<0.01).Conclusions:1 PAR1 expressed mainly in the cutin cell membrane of epidermis with psoriasis vulgaris from basal layer to stratum spinosum, and was obviously higher than the contrast group, and in the active stage it was obviously higher than in the stationary stage. PAR2 expressed mainly in the cutin cell cytoplasm and cell membrane of epidermal granular layer and stratum spinosum with psoriasis vulgaris, and was obviously higher than the contrast group, and in the active stage it was obviously higher than in the stationary stage. It suggested that PAR1 and PAR2 in the angiogenesis with psoriasis vulgaris may play an important role, and was associated with disease progression.2 Compared with normal contrast l group, MVD of psoriasis vulgaris group was obviously higher. Compared with stationary phase, MVD of active stage with psoriasis vulgaris was higher.It suggested that angiogenesis played a role in the pathogenesis of psoriasis, and it’s role in the active stage of psoriasis was more obvious.3 The expression of PAR1, PAR2 was positively correlated in the lesion with psoriasis vulgaris, and the expression of PAR1, PAR2 was positively correlated with microvascular density,suggesting PAR1 and PAR2 may contribute to angiogenesis in lesions with psoriasis vulgaris.