TF?PAR-2?IL-23 Expression And Correlation Studies In Colorectal Cancer

Objective:Our preliminary studies demonstrated that in the surface of the vitro colorectal cancer cell lines SW620,the complexes formed of the TF and VIIa,crosstalk with PAR-2(protein kinaseated receptor-2),and then enhance the proliferation and migration of the cells.Then we made further studies,and found that the TF/VIIa/PAR-2 axis can enhance the proliferation and migration by activating the signal pathways of ERK1/2,NF-kappa B and calcium,and then promote the development of the CRC.Recent studies have found that chronic inflammation promotes the development of malignant tumors.Proinflammatory cytokines play a key role in the initiation,progression,and metastasis of inflammatory related tumors.IL-23,as a proinflammatory cytokine,can be involved in the occurrence and development of autoimmune diseases by NF-kappa B,p38 MAPK,PI3 K and other signaling pathways after binding to the IL-23 receptor.Another preliminary study in our group found that expression of IL-23 was increasing in gastric precancerous lesions and gastric cancer,and IL-23 was expected to be a marker for early diagnosis of gastric cancer.And IL-23 promotes the migration of BGC-823 and SGC-7901 cells by activating the pathway of PI3K/Akt,which shows the role of IL-23 in the progression of chronic gastritis-gastric cancer.Therefore,we intend to observe the expression of TF,PAR-2,and IL-23 in colorectal cancer,and to explore the relationship between them and colorectal cancer.Materials and methods:We choose 60 patients' colon cancer tissue paraffin specimens which was excised by Department of general surgery in Affiliated Hospital of Jiangsu University from June 2015 to August 2016 and was CRC confirmed by surgical pathology as experimental group,and the corresponding adjacent tissues as control group.Immunohistochemical Envision was used to detect the expression of TF,PAR-2,IL-23 in the samples.The general situation of patients and the clinical pathological parameters,chi square test or Fisher exact probability test were used to analyze thecorrelation between the three expressions and the clinical pathological parameters.Results:1.Expression of TF,PAR-2 and IL-23 in CRC and its correlation with clinical pathological parametersCompared with the corresponding adjacent organization,the expression of TF,PAR-2 and IL-23 were higher in CRC,there were significant difference(p<0.0001,p< 0.01,p < 0.0001).The three expressions had no correlation with age,gender,differentiation,pathological type,and so on(p > 0.05).But they had correlations with CRC TNM stage(statistically significant),and lymph node metastasis,but no statistically significant differences;the TF positive expression rate of III/IV was significantly higher than that of I/II(the positive rate of TF were 66.67%,29.63%,p <0.05);The positive rate of TF expression in CRC tissue with lymph node metastasis was higher than that in patients without lymph node metastasis,but the difference was not statistically significant(54.55%,40.74%,p > 0.05);The positive rate of TF expression in CRC tissue with lymph node metastasis was higher than that in patients without lymph node metastasis,but the difference was not statistically significant(54.55%,40.74%,p > 0.05);the PAR-2 positive expression rate of III/IV was significantly higher than that of I/II(respectively 69.70%,33.33%,p < 0.05);The positive rate of PAR-2 expression in CRC tissue with lymph node metastasis was higher than that in patients without lymph node metastasis,but the difference was not statistically significant(respectively 57.58%,44.44%,p > 0.05);the IL-23 positive expression rate of III/IV was significantly higher than that of I/II(respectively66.67%,40.74%,p < 0.05);The positive rate of IL-23 expression in CRC tissue with lymph node metastasis was higher than that in patients without lymph node metastasis,but the difference was not statistically significant(respectively 60.61%,48.15%,p >0.05).2.The expression of TF,PAR-2 and IL-23 correlated with TNM stage and lymph node metastasis of CRCTF,PAR-2 and IL-23 were all positive expression in CRC tissue and the ratiowas 37.5% in I/II,and was 83.33% in III/IV,so III/IV group was higher than that of I/II group,the difference was statistically significant(p < 0.05);The positive rate of TF,PAR-2,IL-23 expression was 60% in CRC tissue with lymph node metastasis was,and was 38.46% in CRC with no lymph node metastasis.The group of lymph node metastasis was higher than that of without lymph node metastasis,but the difference was not statistically significant.Conclusion:1 The expression of TF,PAR-2 and IL-23 in the CRC group was significantly higher than that in the corresponding adjacent tissues,and was related to the later CRC stage of TNM.2 The positive rates of TF,PAR-2 and IL-23 in CRC were correlated with late TNM stage.3.Proinflammatory cytokine interleukin-23,together with TF and PAR-2,are involved in the development of CRC.However,it is necessary to further confirm whether the combination test can contribute to the diagnosis and prognosis of CRC.