Research On The Expression Of Protease Activated Receptors(PARs) And Interleukin-8in The Tissue Prostate Cancer

ObjectiveTo clear the differences of expression of protease activated receptor family and IL-8in the tissue of prostate hyperplasia and prostate cancer, and explore the effects and possible mechanisms of protease activated receptor family and IL-8on the growth of prostate cancer.Methods1.Prostate specimens of patients in department1of urinary surgery of Kunming Medical University from January2012to June2013were collected. Tissues of the prostate cancer group were obtained from prostate puncture, and obtained by B ultrasound guiding transrectal puncture at10. After obtaining, part of the specimens were conserved by microtubes in liquid nitrogen bucket quickly, and then the specimens were stored in the-80℃refrigerator in laboratory for test; The other part were conserved in alcohol with concentration of75%and sent to pathology examination; Tissues of the benign prostatic hyperplasia group were obtained from organization from transurethral prostate hyperplasia electrocision, and cryopreserved method for tissues of prostate hyperplasia was consistent with the prostate cancer group. Two types of tissues were included in the test after obtained and conformed by pathology tests. Peripheral blood PSA values detected by chemiluminescence immunoassay detection method used in nuclear medicine of two groups were both recorded. 2.HE staining images in pathology department were collected, and specimens of two groups were classified and recorded.3.Gene expression of PAR-1,2,4and IL-8in tissues of prostate cancer and hyperplasia was detected by RT-PCR. Protein expression of PAR-1,2,4and IL-8in two types of tissues was detected by Western Blot (Western Blot).Results1.Through pathology examinations, specimens that conformed to the experimental standard in the prostate cancer group were38cases, and the prostate hyperplasia group were35cases. Medical records of specimens of the prostate cancer group were tracked, and making scores according to the Gleason score standard; Staging according to the TNM method of AJCC.38cases of specimens divided into:5cases of T2stage,14cases of T3and17cases of T4. Gleason score system statistics:7cases of Gleason score≤6,5cases of Gleason score=7, and26cases of Gleason score≥8;PSA (0case of0-4pg/ml stage,1case of4-10pg/ml stage,6cases of10.1-20pg/ml stage17.63±2.65pg/ml,31cases of>20pg/ml stage89.66±46.64pg/ml)2.PAR-1,2,4detected by RT-PCR and Western blot were all of high expression in tissues of prostate cancer in different levels, and had statistical significance compared with the BPH group (Table4, P<0.01);3. High expression of IL-8gene and protein level of the prostate cancer group had statistical significance compared with the benign prostate hyperplasia group.(Table5, P<0.05);4. The higher the Gleason score grading of the prostate cancer group, the higher the expression of IL-8gene. There was statistical significance (Table6, P<0.05). The gene expression intensity of IL-8increased with development of prostate cancer staging, and had statistical significance.(Table8, P<0.05)5. The serum PSA value of the prostate cancer group was positively related with Gleason score grading and TNM staging, and had statistical significance.(Table3, P <0.05)Conclusions1. Gene and protein expression of PARs and IL-8in tissues of prostate cancer were all higher than those in tissues of benign prostate hyperplasia;2. The higher the Gleason score grading of the prostate cancer, the higher the gene and protein expression of IL-8;3. The higher the TNM staging of prostate cancer, the higher the gene and protein expression of IL-8;4. The serum PSA value was positively related with Gleason score grading and TNM staging of the prostate cancer group.