Explore The Role Of MALT1and IL-17in The Pathogenesis Of EAE

OBJECTIVE: Multiple sclerosis (MS) which characterized by demyelination inwhite matter of the central nervous system(CNS) is an multiple autoreactiveinflammatory disease. The pathogenesis which is unclear may be related toenvironmental factors and genetic factors. In recent years, more and more scholarsnoted that its pathogenesis are closely related to the nuclear transcription factor-κB(NF-κB) which plays a key role in the process of immune response signaltransduction. Mucosa-associated lymphoid tissue lymphoma transporter1(Mucosaassociated lymphoid tissue lymphoma transport protein1, MALT1) to activate NF-κB.The secretion of were The Th17cells which secretes IL-17(Interleukin-17) have beenshown to play an important role in some autoimmune diseases, infections and otherdiseases. The aim of this experiment is to observe the expression of interleukin-17inserum and MALT1in brain of EAE mice, and to explore their roles in thepathogenesis of EAE. Further explore whether MALTI as multiple sclerosis (MS)therapeutic targets in order to find new means of treatment of MS.METHODS:36C57BL/6female mice were randomly divided into2groups:MOG and complete Freund’s adjuvant use of foam-like mixture preparation EAEmodel group, the adjuvant group only received Freund’s adjuvant. Each animal wasobserved and recorded every two days neurological score once. Six mice wereremoved from two groups randomly after immunization14days,24days,40days.Heart blood and4%paraformaldehyde perfusion brain tissue. Paraffin sections ofbrain tissue from mice, immunehistochemical method to detect MALT1in the mousebrain tissue and IL-17expression in serum by ELISA.RESULTS: Compared with14days and40days EAE group,24days EAE grouphas the highest score of nerve function[（2.5±0.55）vs（0.83±0.41）、（1.5±0.32）p<0.05], weight loss obviously[(17.04±0.41） vs （18.33±0.49）、（18.15±0.13） p<0.05],IL-17concentration in serum [88.0±26.45） vs （122.6±10.11）、（184.4±29.51）p<0.05] and the MALT1expression in brain tissue was also higher[(183.80±9.25）vs（78.25±9.47）、（133.50±19.87）p<0.05]. The Result shows thatthe MALT1expression higher in brain tissue when the neural function score high.CONCLUSIONS: MALT1has Pathogenic effect in the progress of EAE, highexpression of MALT1can increase the nerve function score of EAE mice, Thepathogenic effect of MALT1may be associated with the increased expression of IL-17. The result provides a new way of studying the pathogenesis of EAE andtreatment of multiple sclerosis.