The Expression And Significance Of SOX2and CyclinD1in Epithelial Ovarian Cancer

Ovarian cancer is one of the common malignant tumors in department ofgynecology and has the highest mortality rate in gynecological oncology. Althoughmany domestic and foreign research related to ovarian cancer, its etiology andpathogenesis are not yet clear. For ovarian cancer often has no typical symptoms atthe early stage and lack of early diagnosis index, most patients are final diagnosedwith advanced symptoms, such as ascites and distant metastasis. Although the surgeryand chemotherapy can significantly improve the life quality of patients with advancedovarian cancer, the overall5-year survival rate is still less than46%. At present, theovarian cancer has become a serious threat to the women’s life. Ovarian cancer aremost originated in ovarian epithelial tissue, About75%～90%patients of all ovariancancer was epithelial ovarian cancer(EOC),to study the mechanism of carcinogenesisand development about EOC, looking for effective biomarkers and therapeutic targetsand improving the prognosis of patients are still the difficulty and hotspot in theresearch of ovarian cancer.Sex determining region Y-box2(SOX2) is a stem cell transcription facter,playing an important role in the development of embryo, organization, blood cells,and the nervous system. In recent years, studies have shown that SOX2wereabnormal expression in many malignant tumors, and is closely related to the tumorcell proliferation, invasion and metastasis. At present, the research about the role ofSOX2in EOC is rarely reported at home and abroad, and the results are not same, so still need to further study. CyclinD1as a cell cycle regulation gene, its overexpressioncan make cell proliferation out of control, which has been confirmed to related to thecarcinogenesis and development of many malignant tumors, such as prostate,liver andlung cancer. Through detecting the expression of SOX2and CyclinD1in the tissuesof EOC, discussing the roles of SOX2and CyclinD1in the development of EOC,expecting to find out new theory basis for the early diagnose and therapy of EOC.ObjectiveResearch the expression of SOX2and CyclinD1in different ovarian epithelialtissues, to discuss the effect and significance of them in the process of occurrence anddevelopment of EOC,and to analysis the relationship and significance between themin EOC.Materials and methods1．Study objects83cases of surgical specimens which were collected from thepathology department of The First and Third Affiliated Hospital of ZhengzhouUniversity from August2011to April2013. They were pathologically confirmedincluding43cases of EOC tissues(Malignant Group;26cases of serouscystadenocarcinoma,10cases of mucinous cystadenocarcinoma and7cases ofendometrioid carcinoma),20cases of benign epithelial ovarian neoplasmstissues(Benign Group;10cases of serous cystadenoma and10cases of mucinouscystadenoma) and20cases of normal tissues(Normal Group). The age of them rangedfrom21to79years, the median age was41years. The patients of Malignant Grouphad not received the preoperative radiotherapy or chemotherapy.2．Methods The protein expression of SOX2and CyclinD1in83cases ofepithelial ovarian tissues were detected by Immunohistochemical method. The mRNAexpression of SOX2and CyclinD1in83cases of epithelial ovarian tissues weredetected by RT-PCR method.3．Statistics approach SPSS17.0was used to deal with the data. Chi-squaretest and Fisher’s Exact Test were used to compare the protein expression of SOX2 and CyclinD1in different epithelial ovarian tissues, and to evaluate the relationshipsof them with clinical pathological features, significant level α=0.05; the comparisionbetween two groups, significant level α′=α/comparision times=0.0167. Using thesingle factor analysis of variance to compare the mRNA expression of SOX2andCyclinD1in different epithelial ovarian tissues,comparision between groups using theLSD-t method. The correlation between SOX2protein and CyclinD1protein in EOCwas assessed by Pearson Contingency Coefficient, significant level α=0.05.Results1．SOX2-positive protein was stained in the nucleus. The rate of SOX2-positiveprotein expression was5%(1/20),45.0%(9/20) and81.4%(35/43)in normal, benign,and malignant groups, respectively(P<0.001). Any pairwise comparisons weresignificant variation(P<0.0167).2． CyclinD1-positive protein was stained in the nucleus. The rate ofCyclinD1-positive protein expression was0%(0/20),35.0%(7/20) and69.8%(30/43)in normal, benign, and malignant groups, respectively(P<0.001). Any pairwisecomparisons were significant variation(P<0.0167).3．The expressions of SOX2and CyclinD1protein in EOC was related to FIGOstage, histological differentiation and lymph node metastasis(P<0.05); but not relatedto pathological type(P>0.05).4．The expression quantity of SOX2mRNA was0.109±0.030,0.439±0.104and0.793±0.174in normal, benign, and malignant groups, respectively(P<0.001). Anypairwise comparisons were significant variation(P<0.0167).5．The expression quantity of CyclinD1mRNA was0.072±0.026、0.375±0.097and0.681±0.180in normal, benign, and malignant groups, respectively(P<0.001).Any pairwise comparisons were significant variation(P<0.0167).6．There was positive correlation between SOX2protein and CyclinD1proteinexpression in EOC(rp=0.422，P=0.002). Conclusions1．SOX2was over-expressed in the tissue of EOC, suggesting that SOX2mayinvolved in the process of occurrence and development of EOC.2．CyclinD1was over-expressed in the tissue of EOC, suggesting that SOX2may involved in the process of occurrence and development of EOC.3．The protein exoression of SOX2and CyclinD1in EOC was direct correlation,suggesting that SOX2may be in coordinated with CyclinD1in carcinogenesis andprogression of EOC.