The Study Of Psoriasis Different Syndromes Blood T Lymphocytes Membrane Protein Differences In Membrane Protein Proteomics

ObjectiveResearch of blood T lymphocyte membrane protein differences between patients with psoriasis vulgaris and normol people and in different syndromes of TCM of patients with psoriasis vulgaris. To establish membrane protein/peptide mapping which have differences for Blood T lymphocytes. Clear relationships in function of differential protein and the pathogenesis of psoriasis, syndromes of TCM.MethodsTo collect anticoagulant blood of different syndromes of TCM(blood-heat syndrome, blood-stasis syndrome, blood-dryness syndrome), And extract T lymphocytes and membrane protein. Use clinprotand maldi-tof technology to detect the differential protein.ResultsFound4proteins (Protein peaks:2068.05、1326.51、1230.14、4747.3) i-n normal subjects and patients with psoriasis vulgaris,1protein(7766.6Da)between groups of blood-heat syndrome, and blood-stasis syndrome,1pro tein(7765.96Da)between groups of blood-heat syndrome, and blood dryness s-yndrome.GonelusionIn this study, we find4significant differences membrane protein peaks with proteomics technology between psoriasis vulgaris and normal. In these protein peaks,4747.3Da belong to psoriasis patients, and we speculate that4747.3Da associated with the pathogenesis of psoriasis.We find1significant differences membrane protein peak between blood-stasis syndrome and blood-dryness syndrome, blood-heat syndrome and blood-stasis syndrome. And the2protein peaks are closer, we speculate that 7766Da are belong to both blood-dryness syndrome, blood-heat syndrome.No significant difference was found between blood heat syndrome and dryness,we speculate that there was no significant differences membrane protein between them.