Jiangzhuo Particles Containing Serum Fibroblast Proliferation In Mice

Objective: this topic with serum pharmacology method,obtaining thedrug-containing after giving the rabbit lavage material particles to give the rabbitlavage after obtaining the drug-containing serum, will gather material granuledrug-containing serum on mice fibroblasts in vitro.Based on fibroblast morphologyand cell proliferation were observed in mice, further study of renal fibrosis.Methods: Choose healthy male12only normal rabbit, randomly divided intofour groups (only/group), the normal feeding a week respectively according to therequirements of design of experiment to the feeding material granules.For seven days,twice a day.(1) normal group, saline lavage,10ml/l·d.(2) the low dose group, materialparticle effective dose of2.1g/(kg·d);(3) in the dose group, material particle effectivedose of4.2g/(kg·d);(4) high dose group of material particles effective dose of8.4g/(kg·d).Continuous lavage7days after carotid artery intubation blood collected,centrifugal preparation containing medicine serum, serum after the0.22um film filter,frozen in-80℃low temperature refrigerator.Collect logarithmic phase mice fibroblast,adjust the cell density, vaccination in96-well plates, according to the experiments inlow, medium and high concentration groups each drug-containing serum, controlgroup plus complete medium, concentration of each set set5parallel holes.Observedfibroblasts in the process of cultivation, and respectively in the12h,24h,48h,72hpoint in time, determined by MTT method is used to detect fibroblasts.Each OD valuecalculation, the inhibition rate (IR) of time.Results: Material particles interact between groups medicated serum in micefibroblast, compared with control group, in12h,24h,48h,72h points compared to theOD values are less than the control group, after statistics processing, the differencewas statistically significant (P<0.05);Material particles containing medicine serumtest results of the impact of the cell vitality, each material granule drug-containingserum mice into fiber cell vitality fine12h,24hours inhibit obviously, low dose groupof weakened inhibition effect after48hours;Inhibition rate, high dose group in12h,24h after the suppression effect is abate, cell inhibition rate drop, instructions for cell vigor inhibition rate decreased with the extension of time, after statistics processing,the difference was statistically significant (P<0.05);Each material particles containingmedicine serum at the same time, the inhibition rate with the increase of materialparticles containing medicine serum concentration increases, after statisticsprocessing, the difference was statistically significant (P <0.05).Conclusion: Material granule drug-containing serum groups all can inhibit micefibroblast proliferation, and groups of inhibition rate was most pronounced in12to24hours;Under the same concentration, material particles containing medicine serum ofmice fibroblasts in vitro inhibition rate decreased with the extension of time, at thesame time, the mouse fibroblasts in vitro inhibition rate with the increase of materialparticles containing medicine serum concentration increased， Material granuledrug-containing serum inhibition of cell viability was dose and time dependent.