Jiangzhuo Particles Containing Serum Effect On Murine Renal Tubular Epithelial Cells Of Smads MRNA

Objective: This topic through the normal rabbit serum effect after intragastricadministration of medicated collected in cultured murine renal tubular epithelial cellsin serum pharmacological method to observe Jiangzhuo particles containing serum onmouse renal tubular epithelial cells Smads mRNA the impact of the level of in vitrocell and molecular biology from Jiangzhuo particles explore prevention of renalfibrosis in rats.Methods:10male rabbits were randomly divided into five different groups,using Jiangzhuo particles and benazepril (Lotensin) orally, preparation containingserum.①normal group: given distilled water;②low concentration group:Jiangzhuo particles made of a low concentration of medicine was administered, theeffective dose of particles Jiangzhuo2.1g/(kg d);③concentration group:Jiangzhuo particle system the concentration of the solution fed into medicine,Jiangzhuo particle effective dose4.2g/(kg d);④high concentration group:Jiangzhuo particles made of a high concentration of medicine was administered, theeffective dose of particles Jiangzhuo8.4g/(kg d);⑤benazepril (Lotensin) group:suspension administered benazepril benazepril effective dose of0.467mg/(kg d).Fed the rabbits seven days, at the end of gavage1h neck artery blood was preparedcontaining serum. Tubular epithelial cells of normal mice culture medium waschanged once a day, four days a subculture, when the cells grow to70%confluencewere randomly divided into six different groups to add TGF-β1and serum containingthe experiment24hours after the intervention.Results: after TGF-β1stimulated murine renal tubular epithelial cells lose theirnormal cobblestone shape, contours are not clear, after descending the turbid particles and serum containing benazepril intervention, cell morphology has been restored.⑵Jiangzhuo various concentrations of particles and benazepril group can inhibit theexpression of Smad2mRNA, but low Jiangzhuo particles, the degree of inhibition inconcentrations of less than Smad2mRNA expression benazepril group, the differencewas statistically significant (P <0.05-0.01), high concentrations Jiangzhuo particlesinhibit the expression level of Smad2mRNA and benazepril group rather, thedifference was not statistically significant (P>0.05); Jiangzhuo various concentrationsof particles and benazepril group were raised Smad7mRNA expression, low particleconcentrations Jiangzhuo raised Smad7mRNA expression levels below benazeprilgroup, the difference was statistically significant (P <0.01), the particle concentrationJiangzhuo group and benazepril group comparable level, the difference was notstatistically significant (P>0.05), high concentrations of particulate Jiangzhuo groupthan benazepril group, the difference was statistically significant (P <0.01).Conclusion: Jiangzhuo particles in each dose group can be restored to someextent impaired renal tubular epithelial cell morphology; may inhibit the expression ofSmad2mRNA, but the low concentration group less than benazepril group, the highconcentration group and benazepril Plymouth group considerable role. Jiangzhuovarious concentrations of particles can be raised Smad7mRNA expression, butcompared with benazepril group Jiangzhuo low particle concentrations less thanbenazepril group, middle dose group and benazepril group fairly high concentrationgroup than benazepril group. Jiangzhuo particles probably by inhibiting theexpression of Smad2mRNA, the increase in Smad7mRNA expression to play a rolein prevention and treatment of renal fibrosis.