Changes Of TGFβ₁, TGFβ Receptors And P27 In Benign Prostate Hyperplasia, Prostate In Neoplasm And Prostate Carcinoma

Objectives: To detect the alteration of TGF β₁ , TGFβ receptors and P27 protein in benign prostate hyperplasia(BPH), prostate in neoplasm (PIN) and prostate carcinoma (PCa) and further investigate the role of TGF β signal transduction pathway in prostate tumorigenesis. Some clues for target therapy is expected to be obtained from this study.Methods: Immunohistochemistry method was used to detect the expression of TGF β₁ , TGF β receptors(TGF β Rs) and P27 protein in 28 cases with BPH ,26 cases with PIN and 30 cases with PCa. The images were analyzed by CMIAS system of computer -assisted imaging system and the result was further dealt with spss 11.5 statistical software. Results: 1. The location and expression of four proteins in BPH, PIN, PCa1.1 The location and expression of TGF β₁ protein in BPH, PIN, PCa.Immunoreaction to TGF β₁ in BPH was observed in epithelium, some cells in the connective tissue stroma were also stained. In PCa, the epithelial cell was intensely immunostaised. Some stromal cells were also stained. The expression of TGF β₁ in PCa exhibited a sigficantly increased immunoreactivity compared with PIN and BPH (P<0.01), while no significant difference between PIN and BPH was found in epithelium and stroma (P<0.05).1.2 The location and expression of TGF β R I protein in BPH, PIN, PCaIn BPH and PIN, immunostaining to TGF β R I was found in the whole epithelium and in some stromal cells. In PCa, the immunostaining pattern for thisreceptor was similar to that of PIN. The expression of TGF 3 R I in PCa exhibited a sigficantly reduced immunoreactivity compared with PIN and BPH (P<0.01), while no significant difference between PIN and BPH was found (P>0.05). There is no difference among groups in stroma (P>0.05).1.3 The location and expression of TGF 3 RII protein in BPH, PIN, PCaImmunoreactivity to TGF 3 RII appeared in basal cells and some scattered colummar cell of prostate in BPH. In the PIN sections, this pattern was maintained but less intense in the epithelium (P<0.01), but that of the expression in the stroma was the same as the PIN. TGF 3 RII was also less expressed in PCa than in PIN and BPH in the epithelium and stroma(P<0.01).1.4 The location and expression of P27 protein in BPH, PIN, PCaUniformly strong immunoreactivity of P27 was exclusively localized in the nucleus of epithelium in BPH and PIN , cytoplasm was also stained in PIN. In PCa, P27 staining was in the nucleus and cytoplasm. P27 immunostaining in the whole epithelium decreased from BPH to PIN (P<0.01) and from PIN to PCa(P<0.01), the expression of stroma did not show difference among three groups (P>0.05).2. Correlation between the expression of four proteins and clinico-pathological data in PCa.The expression of TGF 3 i and P27 is significantly correlated with clinical stages (/><0.01). No significant correlation was found between other proteins and clinico-patholigical data. Conclusions:1. In the PIN, deregulation of TGF 3 RII and P27 protein indicated that they were involued in the occurrence of prostate carcinoma and was possibly premalignant precursor in the process of malignant transformation of prostate.2. In this study, we found upregulation of TGF 3 i and deregulation of TGF 3 Rs...