| Objective: The present study is aimed to explore the molecular mechanis ofmyocardial injury protective effect of the experimental animals treated by Tibetanmeidicine Oxytropis falcate bunge in different dose groups, and provide thetheoretical and experimental basis for the treatment of cardiovascular diseases.Methods:120healthy SD rats,(150±20)g body weight, have been grouped andnumbered by male and female respectively for the experiment. Afterwards they havebeen randomly divided into six groups:sham operating group(injected by saline dailyby0.6k/gk), model group (injected by saline daily by0.6k/gk),propranolol controlgroup(10g/kg daily),oxytropis falcate bunge high ethanol extract(15g/kg per day),middle extract(10g/kg per day), low dose extract(5g/kg per day), totally six groups.Before the experiment, the rast were treated by intragastric administration for10days,and the physiological changes were observed daily. The rats were fasted for10hourson the tenth day, and prepared to myocardial ischemia-reperfusion injury model. TheST segment changes have been recorded during ischemia and reperfusion. All theanimals have been treated for30min ischemia,40min reperfusion at the end ofexperiment, and then the heart was removed, washed with ice-cold saline. The apicalpart of heart was selected for the preparation of myocardial biopsy for histologicalexamination, the rest of issues were stored at-80℃, and the Bcl-2and Bax proteinexpression were analysed by ELISA.Results:(1)The myocardial ischemia reperfusion treated by Oxytropis falcate extract,and comparation of ST segment before and after myocardial ischemia-reperfusion, itshows that the ST-segment of sham operating group is no big different compared withmodel group, and ST-segment average elevation in propranolol group and oxytropisfalcate groups are decreased(P<0.05).(2) Resuls from electron microscopy:Compared to model group, the myocardial injury degree of oxytropis falcate groups are significantly lighter than the control group and model group. The myocardialinjury of sham operating group is no abnormal, and myocardial injury level inOxytropis falcate group and the propranolol group are significantly lower (P<0.01).（3）Anti-apoptotic protein Bcl-2expression is reduced and pro-apoptotic Bax proteinis enhanced after myocardial ichemia and reperfusion. After treatment of Oxytropisfalcate extract, compared to the model group, Bcl-2expression is increased indifferent dose group of Oxytropis falcate treatment, and Bax expression is decreased.The most high expression of protein have been found in high dose group, and it issignificantly different (P<0.01).Bcl-2and Bax expression are also no different inpropranolol group and Oxytropis falcate group(P﹥0.01).Conclusion: Apoptosis is found in ischemic reperfusion of myocardial cell, and Bcl-2and bax are invovled in the regulation of myocardial ischemia-reperfusioninjury-induced apoptosis. Oxytropis falcate treatmend will reduce the number ofapoptotic cells, and the mechanism could be related with the increase of Bcl-2proteinexpression and decrease of Bax expression. |
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